In vitro characterization of human dental pulp stem cells isolated by three different methods
| Autor: | Sang Hyuk Park, Mo Kwan Kang, Kyu Min Cho, Euiseong Kim, Hee-Woong Shin, Hyeon-Woo Lee, Ji-Hyun Jang |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
business.industry Mesenchymal stem cell Dental pulp stem cells Isolation method Dentistry 030206 dentistry General Medicine Biology In vitro lcsh:RK1-715 stomatognathic diseases 03 medical and health sciences 030104 developmental biology 0302 clinical medicine stomatognathic system Tissue engineering lcsh:Dentistry Mesenchymal stem cells business Research Article |
| Zdroj: | Restorative Dentistry & Endodontics Restorative Dentistry & Endodontics, Vol 41, Iss 4, Pp 283-295 (2016) |
| ISSN: | 2234-7666 2234-7658 |
| Popis: | Objectives In this study, we characterized human dental pulp cells (HDPCs) obtained by different culture methods to establish the most suitable methodology for dental tissue engineering and regenerative endodontic applications. Materials and Methods HDPCs were isolated by the outgrowth method (HDPCs-OG), the enzymatic digestion method (collagenase/dispase/trypsin, HDPCs-ED), or the combination of both methods (HDPCs-Combined). The expression of mesenchymal stem cell markers (CD105, CD90, and CD73) was investigated. In vitro differentiation capacities of HDPCs into adipogenic, osteogenic, and chondrogenic lineages were compared. Differentiation markers were analyzed by quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blotting. Results Our data indicated that whole HDPCs-ED, HPDCs-OG, and HDPCs-Combined could be differentiated into adipogenic, chrondrogenic, and osteogenic cell types. However, we found that the methods for isolating and culturing HDPCs influence the differentiation capacities of cells. HDPCs-OG and HDPCs-ED were preferably differentiated into adipogenic and osteogenic cells, respectively. Differentiation markers shown by RT-PCR and western blotting analysis were mostly upregulated in the treated groups compared with the control groups. Conclusions Our findings confirmed that cell populations formed by two different culture methods and the combined culture method exhibited different properties. The results of this study could provide an insight into regenerative endodontic treatment using HDPCs. |
| Databáze: | OpenAIRE |
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