Regeneration in vitro of previously frozen adult mouse and human striated muscle coupled with fetal spinal cord
| Autor: | Stanley M. Crain, Edith R. Peterson, M.B. Borstein, J.A. Liveson |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Pathology
medicine.medical_specialty Biology Tissue culture Mice Fetus Developmental Neuroscience Culture Techniques Freezing medicine Animals Humans Regeneration Dimethyl Sulfoxide Process (anatomy) Fetal spinal cord Slow freezing Regeneration (biology) Muscles In vitro Cell biology Neurology Spinal Cord Organ Specificity Ultrastructure Tissue Preservation Explant culture |
| Zdroj: | Experimental neurology. 48(3 Pt 1) |
| ISSN: | 0014-4886 |
| Popis: | Methods were studied to permit freezing of adult mammalian skeletal tissue, storage, thawing, and subsequent explantation and development in organotypic tissue culture. A slow freeze method in the presence of 1M DMSO produced muscle whose regeneration in vitro after thawing was similar to that of unfrozen tissue. No significant ultrastructural or electrophysiological differences were detected between muscle explants which regenerated from frozen and unfrozen tissues. The DMSO appears to protect against gross structural destruction which occurs during the thawing process. A slow freezing rate improved the muscle's regenerative capacity, but the thawing rate had little influence. The morphological correlates and their implications are discussed. |
| Databáze: | OpenAIRE |
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