Trichosporon asahii and Trichosporon inkin Biofilms Produce Antifungal-Tolerant Persister Cells
| Autor: | Reginaldo Gonçalves de Lima-Neto, Lívia Maria Galdino Pereira, Débora de Souza Collares Maia Castelo-Branco, Rossana de Aguiar Cordeiro, José Júlio Costa Sidrim, Ana Luiza Ribeiro Aguiar, Marcos Fábio Gadelha Rocha, Zoilo Pires de Camargo, Fernando Victor Monteiro Portela, Bruno Nascimento da Silva |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Trichosporon spp Multidrug tolerance Chemistry Immunology Biofilm trichosporonosis Trichosporon asahii medicine.disease Antimicrobial Trichosporonosis Microbiology QR1-502 amphotericin B Minimum inhibitory concentration Cellular and Infection Microbiology Infectious Diseases Amphotericin B antifungal susceptibility medicine dormant cells Fungemia Original Research medicine.drug |
| Zdroj: | Frontiers in Cellular and Infection Microbiology Frontiers in Cellular and Infection Microbiology, Vol 11 (2021) |
| ISSN: | 2235-2988 |
| DOI: | 10.3389/fcimb.2021.645812 |
| Popis: | Persister cells are metabolically inactive dormant cells that lie within microbial biofilms. They are phenotypic variants highly tolerant to antimicrobials and, therefore, associated with recalcitrant infections. In the present study, we investigated if Trichosporon asahii and T. inkin are able to produce persister cells. Trichosporon spp. are ubiquitous fungi, commonly found as commensals of the human skin and gut microbiota, and have been increasingly reported as agents of fungemia in immunocompromised patients. Biofilms derived from clinical strains of T asahii (n=5) and T. inkin (n=7) were formed in flat-bottomed microtiter plates and incubated at 35°C for 48 h, treated with 100 μg/ml amphotericin B (AMB) and incubated at 35°C for additional 24 h. Biofilms were scraped from the wells and persister cells were assayed for susceptibility to AMB. Additionally, we investigated if these persister cells were able to generate new biofilms and studied their ultrastructure and AMB susceptibility. Persister cells were detected in both T asahii and T. inkin biofilms and showed tolerance to high doses of AMB (up to 256 times higher than the minimum inhibitory concentration). Persister cells were able to generate biofilms, however they presented reduced biomass and metabolic activity, and reduced tolerance to AMB, in comparison to biofilm growth control. The present study describes the occurrence of persister cells in Trichosporon spp. and suggests their role in the reduced AMB susceptibility of T. asahii and T. inkin biofilms. |
| Databáze: | OpenAIRE |
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