Pumpless Microflow Cytometry Enabled by Viscosity Modulation and Immunobead Labeling
| Autor: | Sang Gu Yim, Su Yeon Shin, Sungyoung Choi, Young Ki Hahn, Seung Yun Yang, Se In Oh, Byeong Yeon Kim |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Staining and Labeling medicine.diagnostic_test Viscosity Chemistry Dynamic range 02 engineering and technology Flow Cytometry 021001 nanoscience & nanotechnology Microspheres Analytical Chemistry Flow cytometry 03 medical and health sciences 030104 developmental biology Modulation medicine Humans Microtechnology Fluidics K562 Cells 0210 nano-technology Cytometry Fluorescent Dyes Biomedical engineering |
| Zdroj: | Analytical Chemistry. 90:8254-8260 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.8b01804 |
| Popis: | Major challenges of miniaturizing flow cytometry include obviating the need for bulky, expensive, and complex pump-based fluidic and laser-based optical systems while retaining the ability to detect target cells based on their unique surface receptors. We addressed these critical challenges by (i) using a viscous liquid additive to control flow rate passively, without external pumping equipment, and (ii) adopting an immunobead assay that can be quantified with a portable fluorescence cell counter based on a blue light-emitting diode. Such novel features enable pumpless microflow cytometry (pFC) analysis by simply dropping a sample solution onto the inlet reservoir of a disposable cell-counting chamber. With our pFC platform, we achieved reliable cell counting over a dynamic range of 9-298 cells/μL. We demonstrated the practical utility of the platform by identifying a type of cancer cell based on CD326, the epithelial cell adhesion molecule. This portable microflow cytometry platform can be applied generally to a range of cell types using immunobeads labeled with specific antibodies, thus making it valuable for cell-based and point-of-care diagnostics. |
| Databáze: | OpenAIRE |
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