Three-dimensional laser scanning two-photon fluorescence confocal microscopy of polymer materials using a new, efficient upconverting fluorophore
| Autor: | W. S. Liou, Guang S. He, J. K. Samarabandu, Jacek Swiatkiewicz, Ping-chin Cheng, Prk Prasad, Ronald Berezney, S. J. Pan, Jayant D. Bhawalkar |
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| Rok vydání: | 2006 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Microscopy Confocal Materials science Polymers business.industry Super-resolution microscopy Lasers Scanning confocal electron microscopy Atomic and Molecular Physics and Optics law.invention Optics Microscopy Fluorescence Two-photon excitation microscopy Confocal microscopy law Light sheet fluorescence microscopy Stilbenes Microscopy Image Processing Computer-Assisted Optoelectronics business Instrumentation Fluorescent Dyes Fluorescence loss in photobleaching |
| Zdroj: | Scanning. 18:562-566 |
| ISSN: | 1932-8745 0161-0457 |
| DOI: | 10.1002/sca.4950180805 |
| Popis: | Three-dimensional confocal imaging of polymer samples was achieved by the use of two-photon excited fluorescence in both positive and negative contrast modes. The fluorophore was a new and highly efficient two-photon induced upconverter, resulting in improved signal strength at low pumping power. Because of the relatively long wavelength of the excitation source (798 nm from a mode-locked Ti:Sap-phire laser), this technique shows a larger penetration depth into the samples than provided by conventional single-photon fluorescence confocal microscopy. Single-photon and two-photon images of the same area of each sample show significant differences. The results suggest the possibility of using two-photon confocal microscopy, in conjunction with highly efficient fluorophores, as a tool to study the surface, interface, and fracture in material science applications. |
| Databáze: | OpenAIRE |
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