Immunogenicity and potential protection of DNA vaccine of Leishmania martiniquensis against Leishmania infection in mice
| Autor: | Benjamas Thanomsub Wongsatayanon, Thuntawat Aunguldee, Piyatida Tangteerawatana, Amporn Jariyapongskul, Orapin Gerdprasert, Saovanee Leelayoova |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_treatment
Leishmaniasis Cutaneous Biology Microbiology DNA vaccination Mice Virology medicine Vaccines DNA Animals Humans Interferon gamma Amastigote Leishmania Mice Inbred BALB C Immunogenicity Vaccination Leishmaniasis General Medicine medicine.disease biology.organism_classification Thailand Interleukin-10 Infectious Diseases Visceral leishmaniasis Leishmaniasis Visceral Parasitology Female Adjuvant medicine.drug |
| Zdroj: | Journal of infection in developing countries. 15(9) |
| ISSN: | 1972-2680 |
| Popis: | Introduction: In Thailand, Leishmania martiniquensis is the predominant species causing cutaneous and visceral leishmaniasis. Its incidence has been increasing among immunocompetent and immunocompromised hosts. We developed a prototype DNA vaccine using a partial consensus sequence of the cysteine protease B (cpb) gene derived from L. martiniquensis from Thai patients. Methodology: The laboratory inbred strain of albino BALB/c mice were immunized intramuscularly three times at 2-week intervals (weeks 0, 2, and 4) with cpb plasmid DNA (pcDNA_cpb) with or without the adjuvant, monoolein (pcDNA_cpb-MO). Mice were challenged at week 6 with L. martiniquensis promastigotes. Sera were analysed for IgG1, IgG2a, interferon gamma and interleukin 10 (IFN-γ and IL-10, respectively) levels at weeks 0, 4, and 9. Additionally, livers and spleens were also analysed for parasite burden using immunohistochemistry and real-time polymerase chain (qPCR) assays. Results: Three weeks after promastigote challenge, vaccinated mice showed significantly increased levels of IgG2a and IFN-γ while IL-10 level was significantly reduced when compared with those in the control group (p < 0.01). Parasite burden in the livers and spleens of vaccinated mice significantly decreased. In addition, a significant increase in mature granuloma formation in the livers when compared with those of the control group (p < 0.05) was found, indicating increased T-helper cells (Th1)-induced inflammation and destruction of amastigotes. Monoolein produced a booster effect to enhance the mouse Th1 protective immunity. Conclusions: The prototype DNA vaccine could induce a Th1 immune response that conferred potential protection to the L. martiniquensis promastigote challenge in BALB/c mice. |
| Databáze: | OpenAIRE |
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