Evaluation of riboflavin and ultraviolet light treatment against Klebsiella pneumoniae in whole blood-derived platelets: A pilot study
| Autor: | Manal Alyousef, Hind Alhumiadan, Mai Alrasheed, Sandra Ramirez-Arcos, Meshari Alabdullatif, Sahar Althawadi, Imad Eldin Osman |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Blood Platelets
Klebsiella pneumoniae Ultraviolet Rays Blood Safety Riboflavin Immunology Pilot Projects Platelet Transfusion 030204 cardiovascular system & hematology Microbiology 03 medical and health sciences 0302 clinical medicine Immunology and Allergy Medicine Humans Platelet Pathogen inactivation Pathogen Whole blood Photosensitizing Agents biology business.industry Inoculation Hematology biology.organism_classification Klebsiella Infections Blood Preservation business Bacteria 030215 immunology |
| Zdroj: | TransfusionREFEREENCES. 61(5) |
| ISSN: | 1537-2995 |
| Popis: | Background Bacterial contamination of platelet concentrates (PCs) is the predominant cause of infectious transfusion reactions. The Pathogen Inactivation Mirasol system was implemented at the King Faisal Specialist Hospital (Saudi Arabia) to reduce the risk of transfusing contaminated PCs. This pilot study evaluated the effectiveness of Mirasol against Klebsiella pneumoniae, a pathogen associated with transfusion reactions, in whole blood-derived PCs. Study design and methods Whole blood (WB) units inoculated with one of six K. pneumoniae strains (five clinical isolates and ATCC-700603) at a concentration of 3-38 CFU/unit, were processed using the platelet-rich plasma (PRP) method. Each spiked PC was pooled with four unspiked units. The pooled PC was split into three Mirasol storage bags: an untreated unit (control), and two units treated with Mirasol at 26 and 32 h post-WB collection, respectively. PC samples obtained before and after Mirasol treatment were used for BacT/ALERT cultures and determination of bacteria quantification. Each experiment was repeated three independent times. Results Five strains were detected prior to PC treatment (24 h post-WB spiking), while one clinical isolate was not detected. Mirasol treatment after 26 h of WB collection resulted in complete inactivation of all K. pneumoniae strains. However, treatment 32 h post-WB collection resulted in the breakthrough of one clinical isolate in two of the three replicates with ~7.8 log10 CFU/unit detected on day 5 of PC storage. Conclusion Delayed Mirasol treatment from 26 to 32 h post-WB collection, resulted in one breakthrough. These results highlight the importance of minimizing the time between WB collection and PI treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text