Measuring Influenza A Virus and Peptide Interaction Using Electrically Controllable DNA Nanolevers
| Autor: | Marlen Kruse, Christin Möser, Ulrich Rant, David M. Smith, Hanna Müller-Landau, Ralph Hölzel, Frank F. Bier |
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| Přispěvatelé: | Publica |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Materials science Peptide 500 Naturwissenschaften und Mathematik::570 Biowissenschaften Biologie::570 Biowissenschaften Biologie multivalency medicine.disease_cause Virology Industrial and Manufacturing Engineering Receptor–ligand kinetics virus-peptide interaction chemistry.chemical_compound chemistry Mechanics of Materials binding kinetics Influenza A virus medicine General Materials Science switchSENSE technology DNA |
| Zdroj: | Advanced Materials Technologies. 7:2101141 |
| ISSN: | 2365-709X |
| Popis: | Electrically controllable deoxyribonuclic acid (DNA) nanolevers are used to investigate the binding interaction between Influenza A/Aichi/2/1968 and the peptide called “PeB”, which specifically binds the viral surface protein hemagglutinin. PeB is immobilized on gold electrodes of a “switchSENSE” biochip by conjugation to DNA-strands that are hybridized to complementary anchors. The surface-tethered DNA strand carries a fluorophore while the complementary strand is a multivalent arrangement carrying up to three PeB peptides. The nanolevers are kept upright (static) by applying a negative potential. Signal read-out for this static measurement mode is the change in fluorescence intensity due to changes in the local environment of the dye upon binding. Measurements of virus-peptide interaction show that the virus material specifically binds to the immobilized peptides and remains bound throughout the measurement time. Immobilized viruses are subsequently used as ligands to characterize oligovalent peptide binding to hemagglutinin, revealing rate constants of the interaction. Moreover, three Influenza A subtypes are compared in their binding behavior. Overall, this paper shows the ability to immobilize virus material on a sensor surface, which allows to target virus-proteins in their native environment. The “switchSENSE” method is therefore applicable to characterize virus-receptor interactions. |
| Databáze: | OpenAIRE |
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