Effective cellulose production by a coculture of Gluconacetobacter xylinus and Lactobacillus mali
| Autor: | Yasuyuki Sasaki, Mayumi Matsushige, Takayasu Tsuchida, Yu Saito, Kenji Ueda, Naoto Tonouchi, Teruhiko Beppu, Akira Seto, Hiroki Kobayashi, Fumihiro Yoshinaga |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Sucrose Molecular Sequence Data Gluconacetobacter xylinus Polysaccharide DNA Ribosomal Zea mays Applied Microbiology and Biotechnology Corn steep liquor Microbiology chemistry.chemical_compound Acetic acid RNA Ribosomal 16S Sequence Homology Nucleic Acid Lactobacillus Cellulose Phylogeny chemistry.chemical_classification Microscopy biology Polysaccharides Bacterial Genes rRNA Sequence Analysis DNA General Medicine Lactobacillaceae biology.organism_classification Culture Media RNA Bacterial chemistry Mutation Microscopy Electron Scanning Bacteria Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 73:915-921 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-006-0515-2 |
| Popis: | A microbial colony that contained a marked amount of cellulose was isolated from vineyard soil. The colony was formed by the associated growth of two bacterial strains: a cellulose-producing acetic acid bacterium (st-60-12) and a lactic acid bacterium (st-20). The 16S rDNA-based taxonomy indicated that st-60-12 belonged to Gluconacetobacter xylinus and st-20 was closely related to Lactobacillus mali. Cocultivation of the two organisms in corn steep liquor/sucrose liquid medium resulted in a threefold higher cellulose yield when compared to the st-60-12 monoculture. A similar enhancement was observed in a coculture with various L. mali strains but not with other Lactobacillus spp. The enhancement of cellulose production was most remarkable when sucrose was supplied as the substrate. L. mali mutants for exocellular polysaccharide (EPS) production were defective in promoting cellulose production, but the addition of EPS to the monoculture of st-60-12 did not affect cellulose productivity. Scanning electron microscopic observation of the coculture revealed frequent association between the st-60-12 and L. mali cells. These results indicate that cell-cell interaction assisted by the EPS-producing L. mali promotes cellulose production in st-60-12. |
| Databáze: | OpenAIRE |
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