A zebrafish mosaic assay to study mammalian stem cells in real time in vivo
| Autor: | Chaoran Yin, Huozhen Hu, Shaohua Yao, Chun Xiao, Meilin Qian, Yonggang Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Mesoderm animal structures Histology Physiology Cellular differentiation Cell Culture Techniques Biology Animals Genetically Modified Mice 03 medical and health sciences 0302 clinical medicine Neural Stem Cells medicine Animals Zebrafish Induced stem cells Tetraploid complementation assay Mosaicism Stem Cells Endoderm Cell Differentiation Cell Biology General Medicine biology.organism_classification Molecular biology Neural stem cell 030104 developmental biology medicine.anatomical_structure Cell Transdifferentiation embryonic structures Stem cell Developmental biology 030217 neurology & neurosurgery |
| Zdroj: | Journal of Molecular Histology. 47:437-444 |
| ISSN: | 1567-2387 1567-2379 |
| Popis: | The differentiation potentials of stem cells have been evaluated by various in vivo and in vitro assays. However, these assays have different limitations hindering efficient study of mammalian stem cells. Here we describe a rapid and powerful mosaic assay to study the differentiation potentials of stem cells in real time in vivo by using zebrafish embryo. We transplanted mouse neural stem cells into zebrafish embryos at different developmental stages and found that they mainly formed neural tissues while occasionally trans-differentiated into mesoderm- and endoderm-derived tissues. Because zebrafish embryo is transparent, the behaviors of transplanted mouse stem cells can be easily tracked in a real-time manner and at single-cell resolution. We expect that this assay may be widely applied to explore the in vivo behaviors of any stem cells available. |
| Databáze: | OpenAIRE |
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