Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis
| Autor: | Valter Sergo, Stefano Fornasaro, Alessandro Esposito, Alois Bonifacio |
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| Přispěvatelé: | Esposito, Alessandro, Bonifacio, Aloi, Sergo, Valter, Fornasaro, Stefano |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Serum
Bioanalysis Materials science Silver label-free SERS biofluids plasmonic paper vibrational spectroscopy serum Clinical Biochemistry biofluid Nanoparticle Metal Nanoparticles Nanotechnology Spectrum Analysis Raman Article symbols.namesake Humans Colloids Plasmon Label free Reproducibility Reproducibility of Results Colloidal silver General Medicine symbols Raman scattering TP248.13-248.65 Localized surface plasmon Biotechnology |
| Zdroj: | Biosensors Biosensors, Vol 11, Iss 467, p 467 (2021) Biosensors; Volume 11; Issue 11; Pages: 467 |
| ISSN: | 2079-6374 |
| Popis: | Label-free SERS is a powerful bio-analytical technique in which molecular fingerprinting is combined with localized surface plasmons (LSPs) on metal surfaces to achieve high sensitivity. Silver and gold colloids are among the most common nanostructured substrates used in SERS, but since protein-rich samples such as serum or plasma can hinder the SERS effect due to protein–substrate interactions, they often require a deproteinization step. Moreover, SERS methods based on metal colloids often suffer from a poor reproducibility. Here, we propose a paper-based SERS sampling method in which unprocessed human serum samples are first soaked on paper strips (0.4 × 2 cm2), and then mixed with colloidal silver nanoparticles by centrifugation to obtain a Centrifugal Silver Plasmonic Paper (CSPP). The CSPP methodology has the potential to become a promising tool in bioanalytical SERS applications: it uses common colloidal substrates but without the need for sample deproteinization, while having a good reproducibility both in terms of overall spectral shape (r > 0.96) and absolute intensity (RSD < 10%). Moreover, this methodology allows SERS analysis more than one month after serum collection on the paper strip, facilitating storage and handling of clinical samples (including shipping from clinical sites to labs). |
| Databáze: | OpenAIRE |
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