A Smaller Form of the Sliding Clamp Subunit of DNA Polymerase III Is Induced by UV Irradiation in Escherichia coli
| Autor: | Tamar Paz-Elizur, Rami Skaliter, Zvi Livneh |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
DNA clamp
Base Sequence biology Ultraviolet Rays DNA polymerase DNA polymerase II Molecular Sequence Data DNA Recombinant DNA replication dnaN Cell Biology Biochemistry DNA polymerase delta Molecular biology DNA polymerase III holoenzyme Enzyme Induction Escherichia coli biology.protein Amino Acid Sequence DNA polymerase I Molecular Biology DNA Polymerase III Plasmids |
| Zdroj: | Journal of Biological Chemistry. 271:2478-2481 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.271.5.2478 |
| Popis: | The beta subunit of DNA polymerase III holoenzyme of Escherichia coli is a 40.6-kDa protein that functions as a sliding DNA clamp (Stukenberg, P. T., Studwell-Vaughan, P. S., and O'Donnell, M. (1991) J. Biol. Chem. 266, 11328-11334). It is responsible for tethering the polymerase to DNA and endowing it with the high processivity required for DNA replication. Here and in a companion study (Paz-Elizur, T., Skaliter, R., Blumenstein, S., and Livneh, Z. (1996) J. Biol. Chem. 271, 2482-2490) we report that the dnaN gene, encoding the beta subunit, contains an internal in-frame gene, termed dnaN*, that encodes a smaller form of the beta subunit. The novel 26-kDa protein, termed beta*, is UV-inducible, and when overexpressed from a plasmid under an inducible promoter, it increases up to 6-fold the UV resistance of E. coli cells. These findings suggest that the beta* protein functions in a reaction associated with DNA repair or recovery of DNA replication in UV-irradiated cells. |
| Databáze: | OpenAIRE |
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