A new approach to epithelial-mesenchymal transition diagnostics in epithelial tumors: double immunofluorescent staining and flow cytometry
| Autor: | Baina K Eliseeva, M. M. Davydov, E. A. Bogush, Sergey A Kaliuzhny, Vladislav Y Kirsanov, A. A. Basharina, V. S. Kosorukov, T. A. Bogush |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Epithelial-Mesenchymal Transition Fluorescent Antibody Technique Vimentin Kaplan-Meier Estimate General Biochemistry Genetics and Molecular Biology Flow cytometry 03 medical and health sciences 0302 clinical medicine medicine Biomarkers Tumor Humans Quantitative expression Epithelial–mesenchymal transition Neoplasms Glandular and Epithelial Solid tumor 030304 developmental biology Proportional Hazards Models Ovarian Neoplasms 0303 health sciences biology medicine.diagnostic_test Chemistry Mesenchymal stem cell medicine.disease Flow Cytometry Progression-Free Survival Staining Gene Expression Regulation Neoplastic 030220 oncology & carcinogenesis biology.protein Keratins Female Single-Cell Analysis Ovarian cancer Biotechnology |
| Zdroj: | BioTechniques. 69(4) |
| ISSN: | 1940-9818 |
| Popis: | A new method of double immunofluorescent staining for flow cytometry has been created to evaluate quantitative expression of mesenchymal protein vimentin only in epithelial cells of a solid tumor that is a mix of different origin cells. De novo vimentin expression is strongly associated with epithelial–mesenchymal transition and therefore is a metastatic potential marker of epithelial tumor cells. In comparison with semiquantitative available methods, the proposed one has several advantages, such as the accurate measurement of the marker’s expression, and minimization of spatial and temporal tumor heterogeneity. Clinical validation of the method has revealed inverse correlation between the quantitative index of epithelial–mesenchymal transition level and progression-free survival using Kaplan–Meier curves and the COX proportional hazards ratio in 32 ovarian cancer patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|