A novel culture method reveals unique neural stem/progenitors in mature porcine iris tissues that differentiate into neuronal and rod photoreceptor-like cells
| Autor: | Daniel Lea, Tamami Matsushita, Masasuke Araki, Shigeru Taketani, Lars N. Royall, Taka-aki Takeda |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Swine Cellular differentiation Sus scrofa Iris Biology 03 medical and health sciences Tissue culture 0302 clinical medicine Neural Stem Cells Retinal Rod Photoreceptor Cells Neurosphere medicine Animals Progenitor cell Molecular Biology Cells Cultured Neurons Matrigel General Neuroscience Cell Differentiation Neural stem cell Cell biology 030104 developmental biology Neurology (clinical) Stem cell 030217 neurology & neurosurgery Fetal bovine serum Developmental Biology |
| Zdroj: | Brain Research. 1675:51-60 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2017.08.027 |
| Popis: | Iris neural stem/progenitor cells from mature porcine eyes were investigated using a new protocol for tissue culture, which consists of dispase treatment and Matrigel embedding. We used a number of culture conditions and found an intense differentiation of neuronal cells from both the iris pigmented epithelial (IPE) cells and the stroma tissue cells. Rod photoreceptor-like cells were also observed but mostly in a later stage of culture. Neuronal differentiation does not require any additives such as fetal bovine serum or FGF2, although FGF2 and IGF2 appeared to promote neural differentiation in the IPE cultures. Furthermore, the stroma-derived cells were able to be maintained in vitro indefinitely. The evolutionary similarity between humans and domestic pigs highlight the potential for this methodology in the modeling of human diseases and characterizing human ocular stem cells. |
| Databáze: | OpenAIRE |
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