Large-scale mapping of transposable element insertion sites using digital encoding of sample identity
| Autor: | Thomas R. Clandinin, Daryl M. Gohl, Marion Silies, Limor Freifeld, Jennifer J. Hwa, Mark Horowitz |
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| Rok vydání: | 2013 |
| Předmět: |
Transposable element
Genetics Genome Insect Chromosome Mapping High-Throughput Nucleotide Sequencing Genomics Sequence Analysis DNA Biology Investigations ENCODE DNA sequencing Set (abstract data type) Drosophila melanogaster Encoding (memory) Identity (object-oriented programming) DNA Transposable Elements Animals Line (text file) |
| Zdroj: | Genetics. 196(3) |
| ISSN: | 1943-2631 |
| Popis: | Determining the genomic locations of transposable elements is a common experimental goal. When mapping large collections of transposon insertions, individualized amplification and sequencing is both time consuming and costly. We describe an approach in which large numbers of insertion lines can be simultaneously mapped in a single DNA sequencing reaction by using digital error-correcting codes to encode line identity in a unique set of barcoded pools. |
| Databáze: | OpenAIRE |
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