Proteomics Research on the Protective Effect of Mangiferin on H9C2 Cell Injury Induced by H2O2
Autor: | Hai-Xue Kuang, Yan-Gang Cheng, Wei Guan, Hong-Liang Ye, Yuan Liu, Qi Wang, Xi-Cheng Jiang, Yan Liu, Bing-You Yang |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Antioxidant
medicine.medical_treatment Pharmaceutical Science Oxidative phosphorylation Pharmacology Fatty acid degradation medicine.disease_cause mangiferin Analytical Chemistry lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine proteomics lcsh:Organic chemistry Drug Discovery medicine oxidative stress Glycolysis cardiovascular diseases Physical and Theoretical Chemistry Mangiferin 030304 developmental biology myocardial ischemia and reperfusion injury 0303 health sciences Fatty acid metabolism Organic Chemistry medicine.disease chemistry iTRAQ Chemistry (miscellaneous) 030220 oncology & carcinogenesis cardiovascular system Molecular Medicine H9C2 Reperfusion injury Oxidative stress |
Zdroj: | Molecules, Vol 24, Iss 10, p 1911 (2019) Molecules Volume 24 Issue 10 |
ISSN: | 1420-3049 |
Popis: | Cardiovascular disease is one of the leading causes of morbidity and mortality worldwide. Mangiferin is a natural glucosylxanthone with antioxidant and anti-inflammatory properties, which has been confirmed to protect cardiac cells from myocardial infarction and myocardial ischemia reperfusion injury (MIRI) however, the underlying mechanism is still unclear. As oxidative stress is a major pathogenesis of MIRI, an H9C2 cell injury induced by hydrogen peroxide (H2O2) was established to simulate MIRI in vitro. Herein, the protective effect of mangiferin against MIRI was evaluated and the isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics was applied to explore the underlying molecular mechanism. In this research, mangiferin markedly ameliorated the oxidative imbalance by increasing the antioxidative capacity of the H9C2 cell. Moreover, proteomics analysis revealed that mangiferin pretreatment brought twenty differently-expressed proteins back to normal, most of which were related to glucose and fatty acid metabolism. Glycolysis, citrate cycle, and fatty acid degradation pathways were highlighted by Kyoto Encyclopedia of Gene and Genomes (KEGG) analysis. Western blot validation of six cardiac metabolism-related proteins were consistent with the proteomics analysis. Taken together, mangiferin protected the cardiomyocytes from MIRI by enhancing the antioxidant capacity and increasing the activities of glycolysis, citrate cycle, and fatty acid degradation pathways. |
Databáze: | OpenAIRE |
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