Molecular characterization of two novel proteins All1122 and Alr0750 of Anabaena PCC 7120 conferring tolerance to multiple abiotic stresses in Escherichia coli
| Autor: | Ruchi Rai, Sonia Sen, Shweta Rai, Shivam Yadav, Lal Chand Rai, Antra Chatterjee, Chhavi Agrawal |
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| Rok vydání: | 2019 |
| Předmět: |
Models
Molecular 0301 basic medicine Protein Conformation Hypothetical protein Gene Expression Cyanobacteria Ligands medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Stress Physiological Escherichia coli Genetics medicine Cloning Molecular Heat-Shock Proteins Phylogeny Abiotic component biology Anabaena Abiotic stress General Medicine biology.organism_classification Complementation 030104 developmental biology Biochemistry 030220 oncology & carcinogenesis Heterologous expression Functional genomics |
| Zdroj: | Gene. 685:230-241 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2018.11.038 |
| Popis: | In- silico and functional genomics approaches have been used to determine cellular functions of two hypothetical proteins All1122 and Alr0750 of Anabaena sp. PCC 7120. Motif analysis and multiple sequence alignment predicted them as typical α/β ATP binding universal stress family protein-A (UspA) with G-(2×)-G-(9×)-G(S/T) as conserved motif. qRT-PCR data under UV-B, NaCl, heat, As, CdCl2, mannitol and methyl viologen registered approximately 1.4 to 4.3 fold induction of all1122 and alr0750 thus confirming their multiple abiotic stress tolerance potential. The recombinant E. coli (BL21) cells harboring All1122 and Alr0750 showed 12-41% and 23-41% better growth respectively over wild type control under said abiotic stresses thus revalidating their stress coping ability. Functional complementation on heterologous expression in UspA mutant E. coli strain LN29MG1655 (ΔuspA::Kan) attested their UspA family membership. This study tempted us to suggest that recombinant Anabaena PCC 7120 over expressing all1122 and alr0750 might contribute to the nitrogen economy in paddy fields experiencing array of abiotic stresses including drought and nutrient limitation. |
| Databáze: | OpenAIRE |
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