ASSESSMENT OF ARGININE 97 AND LYSINE 72 AS DETERMINANTS OF SUBSTRATE SPECIFICITY IN CYTOCHROME P450 2C9 (CYP2C9)
| Autor: | Justin R. Scott, Andrew G. Pearson, Katey Witham, Carwyn Davies, Elizabeth M. J. Gillam, Sandra E. Graham, James J. DeVoss |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Hemeproteins
Models Molecular Diclofenac Hemeprotein Mutant Gene Expression Pharmaceutical Science Ibuprofen Arginine Substrate Specificity chemistry.chemical_compound Naproxen Sequence Homology Nucleic Acid Escherichia coli Animals Humans Binding site Heme Cytochrome P-450 CYP2C9 NADPH-Ferrihemoprotein Reductase Pharmacology Binding Sites Base Sequence biology Lysine Mutagenesis Substrate (chemistry) Active site Cytochrome P450 Peptide Fragments Recombinant Proteins Biochemistry chemistry Mutagenesis Site-Directed biology.protein Aryl Hydrocarbon Hydroxylases Holoenzymes |
| Zdroj: | Drug Metabolism and Disposition. 32:431-436 |
| ISSN: | 1521-009X 0090-9556 |
| DOI: | 10.1124/dmd.32.4.431 |
| Popis: | CYP2C9 is distinguished by a preference for substrates bearing a negative charge at physiological pH. Previous studies have suggested that CYP2C9 residues R97 and K72 may play roles in determining preference for anionic substrates by interaction at the active site or in the access channel. The aim of the present study was to assess the role of these two residues in determining substrate selectivity. R97 and K72 were substituted with negative, uncharged polar and hydrophobic residues using a degenerate polymerase chain reaction-directed strategy. Wild-type and mutant enzymes were expressed in bicistronic format with human cytochrome P450 reductase in Escherichia coli. Mutation of R97 led to a loss of holoenzyme expression for R97A, R97V, R97L, R97T, and R97E mutants. Low levels of hemoprotein were detected for R97Q, R97K, R97I, and R97P mutants. Significant apoenzyme was observed, suggesting that heme insertion or protein stability was compromised in R97 mutants. These observations are consistent with a structural role for R97 in addition to any role in substrate binding. By contrast, all K72 mutants examined (K72E, K72Q, K72V, and K72L) could be expressed as hemoprotein at levels comparable to wild-type. Type I binding spectra were obtained with wild-type and K72 mutants using diclofenac and ibuprofen. Mutation of K72 had little or no effect on the interaction with these substrates, arguing against a critical role in determining substrate specificity. Thus, neither residue appears to play a role in determining substrate specificity, but a structural role for R97 can be proposed consistent with recently published crystallographic data for CYP2C9 and CYP2C5. |
| Databáze: | OpenAIRE |
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