Modulation of gene expression by high and low density lipoproteins in human vascular smooth muscle cells
| Autor: | Fabrizia Ferracin, Fritz R. Bühler, Alfred W. A. Hahn, Alfred Pletscher |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Vascular smooth muscle Transcription Genetic Genes myc Biophysics Gene Expression Genes MHC Class I Biology Biochemistry Muscle Smooth Vascular Pathogenesis chemistry.chemical_compound Transforming Growth Factor beta Proto-Oncogene Proteins Internal medicine Proto-Oncogenes Gene expression medicine Humans RNA Messenger Molecular Biology Cells Cultured Lipoproteins HDL3 Arteries Cell Biology Arteriosclerosis Transforming growth factor beta Protein-Tyrosine Kinases Blotting Northern medicine.disease Lipoproteins LDL Kinetics Endocrinology chemistry Low-density lipoprotein biology.protein lipids (amino acids peptides and proteins) Lipoproteins HDL Proto-Oncogene Proteins c-fos Transforming growth factor Lipoprotein |
| Zdroj: | Biochemical and Biophysical Research Communications. 178:1465-1471 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(91)91058-k |
| Popis: | Low density lipoprotein and its oxidized form has been implicated in the process of arteriosclerosis which involves growth-related events in the smooth muscle cells of the arterial wall. The induction of so-called early-growth response genes e.g. c-myc and c-fos can serve as an indicator for these growth-related events. In cultured human vascular smooth muscle cells, both LDL and HDL3 were individually capable of stimulating c-myc and c-fos expression in a concentration dependent manner. However, when they were used in combination, depending on the proportion of HDL3 to LDL, c-fos but not c-myc expression was less pronounced than with the single components. In contrast to HDL3 and LDL alone, a combination of the two lipoproteins also blunted both the expression of autoinduced transforming growth factor beta transcripts and the transforming growth factor beta-induced increase of c-fos mRNA. It is concluded that a) the inhibition of transforming growth factor beta autostimulation by HDL3 plus LDL may involve reduced AP-1 activity via a reduction of c-fos expression by the lipoprotein combination and b) the ratio HDL3:LDL might influence the pathogenesis of arteriosclerosis via growth-related events in the arterial wall. |
| Databáze: | OpenAIRE |
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