Regulation of adiponectin on lipid metabolism in large yellow croaker (Larimichthys crocea)
| Autor: | Renlei Ji, Si Zhu, Qinghui Ai, Kangsen Mai, Xiang Xu, Yongnan Li, Xiaojun Xiang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
animal structures Adipose tissue 030209 endocrinology & metabolism Energy homeostasis Cell membrane 03 medical and health sciences 0302 clinical medicine medicine Larimichthys crocea Animals Receptor Molecular Biology Adiponectin receptor 1 Adiponectin biology Chemistry Lipid metabolism Cell Biology biology.organism_classification Lipid Metabolism Cell biology Perciformes 030104 developmental biology medicine.anatomical_structure Receptors Adiponectin hormones hormone substitutes and hormone antagonists |
| Zdroj: | Biochimica et biophysica acta. Molecular and cell biology of lipids. 1865(8) |
| ISSN: | 1879-2618 |
| Popis: | Adiponectin (APN), an adipose tissue-derived hormone, plays a key role in regulating energy metabolism in mammals. However, its physiological roles in teleosts remain poorly understood. In the present study, the apn gene was cloned from large yellow croaker, which was mainly expressed in the adipose, muscle and liver. Further studies showed that adaptor protein phosphotyrosine interaction PH domain and leucine zipper 1 (APPL1) was localized in the cytoplasm near the cell membrane and was directly bounded to adiponectin receptors (AdipoRs). Meanwhile, APN played a crucial role in lipid metabolism of primary muscle cells by promoting the synthesis, oxidation and transport of fatty acids, and the promoting effects were blocked by knockdown of appl1 and AdipoRs. Furthermore, the activation/inhibition of peroxisome proliferators activated receptor γ (PPARγ) enhanced/suppressed the APN-mediated lipid metabolism. Overall, results showed that APN mediated lipid metabolism through AdipoRs-APPL1 activated PPARγ and further regulated the synthesis, oxidation and transport of FA. This study will facilitate the investigation of APN functions in lipid metabolism and energy homeostasis and reveal the evolution of lipids utilization and energy homeostasis in vertebrates. |
| Databáze: | OpenAIRE |
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