Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells
| Autor: | Saki Kawai, Sho Aoki, Yoko Abe, Rie Imataki, Kenji Arita, Masae Okuno, Kyoko Harada |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Homeobox protein NANOG
Technology QH301-705.5 QC1-999 Population regenerative medicine Regenerative medicine SOX2 Tissue engineering stomatognathic system General Materials Science Biology (General) education Instrumentation QD1-999 Fluid Flow and Transfer Processes education.field_of_study Chemistry Process Chemistry and Technology Physics Mesenchymal stem cell General Engineering Engineering (General). Civil engineering (General) Computer Science Applications Cell biology non-thermal atmospheric pressure plasma (NTAPP) human deciduous dental pulp fibroblast-like cells stomatognathic diseases Stem cell TA1-2040 Adult stem cell |
| Zdroj: | Applied Sciences, Vol 11, Iss 10119, p 10119 (2021) Applied Sciences Volume 11 Issue 21 |
| ISSN: | 2076-3417 |
| Popis: | Human mesenchymal stem cells can differentiate into various cell types and are useful for applications in regenerative medicine. Previous studies indicated that dental pulp exfoliated from deciduous teeth is a valuable alternative for dental tissue engineering because it contains stem cells with a relatively high proliferation rate. For clinical application, it is necessary to rapidly obtain a sufficient number of cells in vitro and maintain their undifferentiated state however, the abundance of stem cells in the dental pulp tissue is limited. Non-thermal atmospheric pressure plasma (NTAPP) has been applied in regenerative medicine because it activates cell proliferation. Here, we examined the effects of NTAPP to activate the proliferation of human deciduous dental pulp fibroblast-like cells (hDDPFs) in vitro. Compared with untreated cells, NTAPP increased cell proliferation by 1.3-fold, significantly upregulated well-known pluripotent genes for stemness (e.g., Oct4, Sox2, and Nanog), and activated the expression of stem cell-specific surface markers (e.g., CD105). Overall, NTAPP activated the proliferation of various mesodermal-derived human adult stem cells while maintaining their pluripotency and stemness. In conclusion, NTAPP is a potential tool to expand the population of various adult stem cells in vitro for medical applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|