Biochemical and immunocytochemical analysis of a histone H1 variant from the mouse testis
| Autor: | Virginia E. Papaioannou, B. K. A. Rasheed, Y. M. Bhatnagar, E. C. Whisenant, Rajesh Ghai |
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| Předmět: |
In situ
Male Cell type Somatic cell Biology Kidney Mouse Testis Histones Mice Histone H1 Testis medicine Animals Tissue Distribution Spermatogenesis chemistry.chemical_classification Brain Genetic Variation Cell Biology Sertoli cell Molecular biology Immunohistochemistry Amino acid medicine.anatomical_structure chemistry Liver Hepatocyte Spleen |
| Zdroj: | Europe PubMed Central |
| Popis: | An H1 histone variant, H1a, has been isolated and purified from the mouse testis. Biochemical and amino acid analyses indicate its similarity with the rat testis H1a. Specific antibodies against the purified H1a have been generated in rabbits and used to study its tissue and species distribution using protein blotting procedures. We have also used the immunocytochemical technique to determine in situ distribution of H1a in spermatogenic cells and somatic tissues of the mouse. A non-random distribution of H1a has been noted in the nuclei of certain somatic cell types such as Sertoli cells, Leydig cells and neurons. By contrast, hepatocyte nuclei lacked detectable levels of H1a. In adult seminiferous tubules, the early primary spermatocyte nuclei displayed a greater level of immunoreactivity relative to other cell types. Developmental studies indicate its initial expression in the 7-day-old mouse testis concomitant with the appearance of intermediate and type B spermatogonia. |
| Databáze: | OpenAIRE |
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