Biocompatibility of Polymer and Ceramic CAD/CAM Materials with Human Gingival Fibroblasts (HGFs)
| Autor: | José-Luis Gutiérre-Pérez, María Rizo-Gorrita, María-Ángeles Serrera-Figallo, Cristina Herráez-Galindo, Daniel Torres-Lagares |
|---|---|
| Přispěvatelé: | Universidad de Sevilla. Departamento de Estomatología |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Polymers and Plastics
Biocompatibility Polymethyl methacrylate (PMMA) Collagen secretion collagen type I fibroblasts/cytology 02 engineering and technology polymethyl methacrylate (PMMA) CAD-CAM cell survival Article Cell survival lcsh:QD241-441 03 medical and health sciences 0302 clinical medicine lcsh:Organic chemistry Collagen type I Cubic zirconia Viability assay Ceramic Silicates/chemistry materials testing biocompatible materials/chemistry Cytotoxicity chemistry.chemical_classification Collagen type Biocompatible materials/chemistry Chemistry 030206 dentistry General Chemistry Polymer 021001 nanoscience & nanotechnology silicates/chemistry Fibroblasts/cytology visual_art visual_art.visual_art_medium 0210 nano-technology Materials testing Nuclear chemistry |
| Zdroj: | Polymers Volume 11 Issue 9 idUS: Depósito de Investigación de la Universidad de Sevilla Universidad de Sevilla (US) Polymers, Vol 11, Iss 9, p 1446 (2019) idUS. Depósito de Investigación de la Universidad de Sevilla instname |
| ISSN: | 2073-4360 |
| DOI: | 10.3390/polym11091446 |
| Popis: | Four polymer and ceramic computer-aided design/computer-aided manufacturing (CAD/CAM) materials from different manufacturers (VITA CAD-Temp (polymethyl methacrylate, PMMA), Celtra Duo (zirconia-reinforced lithium silicate ceramic, ZLS), IPS e.max CAD (lithium disilicate (LS2)), and VITA YZ (yttrium-tetragonal zirconia polycrystal, Y-TZP)) were tested to evaluate the cytotoxic effects and collagen type I secretions on human gingival fibroblasts (HGFs). A total of 160 disc-shaped samples (Ø 10 ± 2 mm h: 2 mm) were milled from commercial blanks and blocks. Direct-contact cytotoxicity assays were evaluated at 24, 48, and 72 h, and collagen type I (COL1) secretions were analysed by cell-based ELISA at 24 and 72 h. Both experiments revealed statistically significant differences (p < 0.05). At 24 and 48 h of contact, cytotoxic potential was observed for all materials. Later, at 72 h, all groups reached biologically acceptable levels. LS2 showed the best results regarding cell viability and collagen secretion in all of the time evaluations, while Y-TZP and ZLS revealed intermediate results, and PMMA exhibited the lowest values in both experiments. At 72 h, all groups showed sharp decreases in COL1 secretion regarding the 24-h values. According to the results obtained and the limitations of the present in vitro study, it may be concluded that the ceramic materials revealed a better cell response than the polymers. Nevertheless, further studies are needed to consolidate these findings and thus extrapolate the results into clinical practice. |
| Databáze: | OpenAIRE |
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