Molecular mechanisms of the naringin low uptake by intestinal Caco-2 cells
| Autor: | Meryl Hassan, O. Ghiringhelli, Marie-Josèphe Amiot, Franck Tourniaire, Marc André, Christian Alquier |
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| Přispěvatelé: | Institut Méditerranéen de Biochimie et Biologie de la Nutrition (IMRN), Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2005 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Permeability Intestinal absorption chemistry.chemical_compound Humans ATP Binding Cassette Transporter Subfamily B Member 1 Intestinal Mucosa Naringin P-glycoprotein Monocarboxylate transporter biology Chemistry CACO-2 Glucose transporter Biological Transport ABSORPTION INTESTINALE Intestinal Absorption Verapamil Biochemistry Caco-2 Flavanones biology.protein Efflux Caco-2 Cells Carrier Proteins Flavanone Food Science Biotechnology |
| Zdroj: | Molecular Nutrition and Food Research Molecular Nutrition and Food Research, Wiley-VCH Verlag, 2005, 49, pp.957-962. ⟨10.1002/mnfr.200500088⟩ |
| ISSN: | 1613-4133 1613-4125 |
| Popis: | Naringin, the main flavanone of grapefruit, was reported to display numerous biological effects: antioxidant, hypocholesteremic, anti-atherogenic and favoring drug absorption. Naringin absorption mechanisms were studied in Caco-2 cells (TC7 clone). We investigated the possible involvement of several membrane transporters implicated in polyphenolic compounds intestinal transport (sodium-dependent glucose transporter 1, monocarboxylate transporter, multidrug-associated resistance proteins 1 and 2, and P-glycoprotein). Naringin was poorly absorbed by Caco-2 cells, according to its low value of apparent permeability coefficient (P(app) = 8.1 +/- 0.9 x 10(-8) cm/s). In the presence of verapamil, a specific inhibitor of P-glycoprotein, cellular uptake was increased by almost threefold after 5 min, and P(app) was doubled after 30 min. Our results indicated the involvement of P-glycoprotein, an ATP-driven efflux pump, capable of transporting naringin from the Caco-2 cell to the apical side. This phenomenon could explain, at least in part, the low absorption of this flavanone at the upper intestinal level. |
| Databáze: | OpenAIRE |
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