Rapid changes in tissue mechanics regulate cell behaviour in the developing embryonic brain
| Autor: | Amelia J Thompson, Eva K Pillai, Ivan B Dimov, Sarah K Foster, Christine E Holt, Kristian Franze |
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| Přispěvatelé: | Thompson, Amelia J [0000-0002-3912-3652], Franze, Kristian [0000-0002-8425-7297], Apollo - University of Cambridge Repository |
| Rok vydání: | 2019 |
| Předmět: |
musculoskeletal diseases
Retinal Ganglion Cells animal structures Embryo Nonmammalian QH301-705.5 Science durotaxis Mitosis Cell Count macromolecular substances developmental biology stiffness Xenopus laevis physics of living systems Animals Optic Tract Biology (General) mechanotransduction atomic force microscopy axon guidance technology industry and agriculture Brain xenopus Axons Biomechanical Phenomena body regions Cell Body Medicine mechanics |
| Zdroj: | eLife, Vol 8 (2019) |
| DOI: | 10.17863/cam.35779 |
| Popis: | Tissue mechanics is important for development; however, the spatio-temporal dynamics of in vivo tissue stiffness is still poorly understood. We here developed tiv-AFM, combining time-lapse in vivo atomic force microscopy with upright fluorescence imaging of embryonic tissue, to show that during development local tissue stiffness changes significantly within tens of minutes. Within this time frame, a stiffness gradient arose in the developing Xenopus brain, and retinal ganglion cell axons turned to follow this gradient. Changes in local tissue stiffness were largely governed by cell proliferation, as perturbation of mitosis diminished both the stiffness gradient and the caudal turn of axons found in control brains. Hence, we identified a close relationship between the dynamics of tissue mechanics and developmental processes, underpinning the importance of time-resolved stiffness measurements. |
| Databáze: | OpenAIRE |
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