Effect of human plasmalipoproteins on erythropoietic progenitor cells in serum-free cultures
| Autor: | H.-J. Lisch, Ch. Breier, Herbert Braunsteiner, C. Peschel, Dietmar Geissler, Günther Konwalinka |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
medicine.medical_specialty
Very low-density lipoprotein Erythrocytes Lipoproteins Lipoproteins VLDL Internal medicine Blood plasma medicine Humans Bovine serum albumin Erythropoietin chemistry.chemical_classification biology Dose-Response Relationship Drug Stem Cells Hematology General Medicine Culture Media Lipoproteins LDL Agar Endocrinology chemistry Transferrin Cell culture biology.protein Erythropoiesis lipids (amino acids peptides and proteins) Lipoproteins HDL medicine.drug Lipoprotein |
| Zdroj: | Blut. 52(3) |
| ISSN: | 0006-5242 |
| Popis: | The purpose of the present study was to investigate the influence of human lipoproteins on CFU-e and BFU-e proliferation from human bone marrow in a serum-free system. In our previously described miniaturized agar system the main lipoprotein-density-classes from human plasma, namely very low density lipoproteins (VLDL), intermediate density lipoproteins (IDL), low density lipoproteins (LDL), high density lipoproteins2 (HDL2) and HDL3 and a mixture of all the five lipoproteins were added in rising concentrations (from 1/10 to normal human plasma concentration) to serum-free medium containing delipidated and deionized bovine serum albumin (BSA), iron saturated transferrin and erythropoietin. The results demonstrate that all lipoproteins markedly increased the CFU-e and BFU-e proliferation after 7 and 14 days of incubation, respectively. Moreover, the lipoproteins induced a shift towards a lower threshold concentration of erythropoietin. Serumlike conditions were obtained if LDL and the mixture of lipoproteins were added to serum-free medium. Furthermore, in the serum-free cultures a maturation to the mature erythrocyte could be found. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|