EBV Infection Is Common in Gingival Epithelial Cells of the Periodontium and Worsens during Chronic Periodontitis
| Autor: | Alain Doglio, Isabelle Prêcheur, Caroline C. Mouline, Patrick Mahler, Janet L. Maryanski, Séverine Vincent-Bugnas, Yves Charbit, Paul Hofman, Wafa Khaali, Sébastien Vitale |
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| Přispěvatelé: | Microbiologie Orale, Immunothérapie et Santé (MICORALIS), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Laboratoire de Pathologie Clinique et Expérimentale. Hôpital Pasteur [Nice], Hôpital Pasteur [Nice] (CHU), Unité de Thérapie Cellulaire et Génique [CHU Nice] (UTCG), Hôpital Pasteur [Nice] (CHU)-Centre Hospitalier Universitaire de Nice (CHU Nice), Centre population et développement (CEPED - UMR_D 196), Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5), Université Henri Poincaré - Nancy 1 (UHP), Université Nice Sophia Antipolis - Faculté de Chirurgie Dentaire (UNS UFR Odontologie), This work was supported in part by the Centre Hospitalier de Nice [AOI N°2010-A01119-30 to SVB] and the Agence Nationale de Recherche contre le SIDA [ANRS N° 10338 to AD]. SV was the recipient of a fellowship from Sidaction, and CCM was the recipient of a fellowship from the Agence Nationale de Recherche [ANR-2010-RFCS-007-02 to AD]., The authors aknowledge the IRCAN Cellular and Molecular Imaging (PICMI) platform, the Institut Fédératif de Recherche (IFR50), the GEPITO laboratory, and the Laboratoire d'Anatomie Cytologie Pathologique du CHU de Nice for helpful access to facilities and equipment for confocal microscopy, microscopy, qPCR and cytocentrifugation, respectively. We thank the statistical department from the CHU de Nice (Eric Fontas) for helpful guidance and interpretation of statistical analysis. We are very grateful to S. Gottshalk and C.Rooney for gift of recombinant Ad5F35 vectors and for helpful discussion., Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Periodontium Chemokine Epstein-Barr Virus Infections MESH: Epithelial Cells/virology [SDV]Life Sciences [q-bio] Gingiva Apoptosis Epithelial cells medicine.disease_cause Epithelium 0302 clinical medicine hemic and lymphatic diseases MESH: Reverse Transcriptase Polymerase Chain Reaction MESH: Aged 0303 health sciences Multidisciplinary MESH: Middle Aged biology Reverse Transcriptase Polymerase Chain Reaction Middle Aged MESH: Chronic Periodontitis/virology Cell staining 3. Good health MESH: Gingiva/virology MESH: Epstein-Barr Virus Infections/virology Medicine Female Research Article Science In Vitro Techniques 03 medical and health sciences medicine Humans Epstein-Barr virus Viral shedding Periodontitis Epstein–Barr virus infection 030304 developmental biology Aged MESH: In Vitro Techniques B cells Reverse transcriptase-polymerase chain reaction MESH: Humans 030206 dentistry medicine.disease Epstein–Barr virus Chronic periodontitis MESH: Male CCL20 Immunology Chronic Periodontitis biology.protein MESH: Female MESH: Periodontium/virology |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2013, 8 (12), pp.e80336. ⟨10.1371/journal.pone.0080336⟩ PLoS ONE, Vol 8, Iss 12, p e80336 (2013) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0080336⟩ |
| Popis: | International audience; An amplifying role for oral epithelial cells (ECs) in Epstein-Barr Virus (EBV) infection has been postulated to explain oral viral shedding. However, while lytic or latent EBV infections of oro/nasopharyngeal ECs are commonly detected under pathological conditions, detection of EBV-infected ECs in healthy conditions is very rare. In this study, a simple non-surgical tissue sampling procedure was used to investigate EBV infection in the periodontal epithelium that surrounds and attaches teeth to the gingiva. Surprisingly, we observed that the gingival ECs of the periodontium (pECs) are commonly infected with EBV and may serve as an important oral reservoir of latently EBV-infected cells. We also found that the basal level of epithelial EBV-infection is significantly increased in chronic periodontitis, a common inflammatory disease that undermines the integrity of tooth-supporting tissues. Moreover, the level of EBV infection was found to correlate with disease severity. In inflamed tissues, EBV-infected pECs appear to be prone to apoptosis and to produce larger amounts of CCL20, a pivotal inflammatory chemokine that controls tissue infiltration by immune cells. Our discovery that the periodontal epithelium is a major site of latent EBV infection sheds a new light on EBV persistence in healthy carriers and on the role of this ubiquitous virus in periodontitis. Moreover, the identification of this easily accessible site of latent infection may encourage new approaches to investigate and monitor other EBV-associated disorders. |
| Databáze: | OpenAIRE |
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