Icariin enhances endothelial nitric-oxide synthase expression on human endothelial cells in vitro
| Autor: | Zhao-Quan Huang, Hai-Bin Xu |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Nitric Oxide Synthase Type III
Physiology Blotting Western Pharmacology Nitric Oxide Gene Expression Regulation Enzymologic Umbilical vein Nitric oxide chemistry.chemical_compound Superoxides Enos Humans RNA Messenger Cells Cultured Flavonoids Regulation of gene expression biology Reverse Transcriptase Polymerase Chain Reaction NF-kappa B Endothelial Cells NF-κB biology.organism_classification In vitro Blot chemistry Biochemistry Molecular Medicine Icariin |
| Zdroj: | Vascular Pharmacology. 47:18-24 |
| ISSN: | 1537-1891 |
| DOI: | 10.1016/j.vph.2007.03.002 |
| Popis: | Icariin, a flavonoid isolated from Epimedii herba, is considered to be the major therapeutical constituent of E. herba. The aim of this study was to investigate the possible protective effects and to clarify the mechanism of icariin on endothelial cells in vitro. Incubation of human umbilical vein endothelial cells (HUVEC) derived EA. hy926 cells with icariin(0.1, 1, 10 micromol l(-1)) from 6 h to 72 h, then the production of NO was measured to evaluate the protective effects of icariin. RT-PCR was employed to confirm the mRNA expression of endothelial nitric oxide synthase (eNOS). Western blotting was used to evaluate the protein expression of eNOS. NO production was enhanced in a time- and concentration-dependent manner (P |
| Databáze: | OpenAIRE |
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