Differential Effects of Dry Eye Disorders on Metabolomic Profile by 1H Nuclear Magnetic Resonance Spectroscopy
Autor: | Manuel Díaz-Llopis, Bárbara Vivar-Llopis, Sebastián Martínez-Castillo, José Manuel Morales, Daniel Monleon, Maria D Pinazo-Duran, C. Galbis-Estrada |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
Adult
Male medicine.medical_specialty Pathology Magnetic Resonance Spectroscopy Arginine Article Subject lcsh:Medicine Creatine General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Metabolomics Ophthalmology medicine Choline Humans Aged Aged 80 and over General Immunology and Microbiology business.industry lcsh:R Pulse sequence General Medicine Nuclear magnetic resonance spectroscopy Middle Aged chemistry Tears Reflex Metabolome Dry Eye Syndromes Female business Research Article |
Zdroj: | BioMed Research International, Vol 2014 (2014) BioMed Research International |
ISSN: | 2314-6133 |
DOI: | 10.1155/2014/542549 |
Popis: | We used1H NMR spectroscopy to analyze the metabolomic profile of reflex tears from patients with dry eye disorders (DEDs). 90 subjects were divided into 2 groups: (1) patients with DEDs (DEDG;n = 55) and (2) healthy subjects (CG;n = 35). Additionally, the DEDG was subdivided into 2 subgroups based on DED severity: mild-to-moderate and moderate (n = 22andn = 33, resp.). Personal interviews and systematized ophthalmologic examinations were carried out. Reflex tears (20–30 μL) were collected by gently rubbing in the inferior meniscus of both eyelids with a microglass pipette and stored at −80°C until analysis. NMR spectra were acquired using a standard one-dimensional pulse sequence with water suppression. Data were processed and transferred to MATLAB for further chemometric analysis. Main differences in tear composition between DEDG and CG were found in cholesterol,N-acetylglucosamine, glutamate, creatine, amino-n-butyrate, choline, acetylcholine, arginine, phosphoethanolamine, glucose, and phenylalanine levels. This metabolic fingerprint helped also to discriminate between the three additional subgroups of DEDG. Our results suggest that tear metabolic differences between DEDG and CG identified by NMR could be useful in understanding ocular surface pathogenesis and improving biotherapy. |
Databáze: | OpenAIRE |
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