Transcriptomics analysis defines global cellular response of Agrobacterium tumefaciens 5A to arsenite exposure regulated through the histidine kinases PhoR and AioS
| Autor: | Brian Bothner, Yoon-Suk Kang, Gejiao Wang, Rachel A. Rawle, Timothy R. McDermott |
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| Rok vydání: | 2018 |
| Předmět: |
Histidine Kinase
Arsenites chemistry.chemical_element Microbiology Gene Expression Regulation Enzymologic Phosphates 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Histidine Ecology Evolution Behavior and Systematics Arsenic 030304 developmental biology Arsenite Regulation of gene expression 0303 health sciences Arsenic toxicity biology 030306 microbiology Kinase Gene Expression Profiling Agrobacterium tumefaciens Gene Expression Regulation Bacterial biology.organism_classification Arsenic contamination of groundwater chemistry Biochemistry Signal transduction Oxidoreductases Oxidation-Reduction |
| Zdroj: | Environmental microbiology. 21(8) |
| ISSN: | 1462-2920 |
| Popis: | In environments where arsenic and microbes coexist, microbes are the principal drivers of arsenic speciation, which directly affects bioavailability, toxicity and bioaccumulation. Speciation reactions influence arsenic behaviour in environmental systems, directly affecting human and agricultural exposures. Arsenite oxidation decreases arsenic toxicity and mobility in the environment, and therefore understanding its regulation and overall influence on cellular metabolism is of significant interest. The arsenite oxidase (AioBA) is regulated by a three-component signal transduction system AioXSR, which is in turn regulated by the phosphate stress response, with PhoR acting as the master regulator. Using RNA-sequencing, we characterized the global effects of arsenite on gene expression in Agrobacterium tumefaciens 5A. To further elucidate regulatory controls, mutant strains for histidine kinases PhoR and AioS were employed, and illustrate that in addition to arsenic metabolism, a host of other functional responses are regulated in parallel. Impacted functions include arsenic and phosphate metabolism, carbohydrate metabolism, solute transport systems and iron metabolism, in addition to others. These findings contribute significantly to the current understanding of the metabolic impact and genetic circuitry involved during arsenite exposure in bacteria. This informs how arsenic contamination will impact microbial activities involving several biogeochemical cycles in nature. |
| Databáze: | OpenAIRE |
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