Characteristics of Clusters of Salmonella and Escherichia coli O157 Detected by Pulsed-Field Gel Electrophoresis that Predict Identification of Outbreaks

Autor: Kia Crocker, Hillary Booth, Joshua Rounds, Quyen Phan, Timothy F. Jones, Nupur Sashti, Shaun Cosgrove, Cyndy Nicholson, L. Hannah Gould, Amanda Ingram
Rok vydání: 2016
Předmět:
0301 basic medicine
Salmonella
Food Safety
030106 microbiology
Biology
Escherichia coli O157
medicine.disease_cause
Models
Biological
Applied Microbiology and Biotechnology
Microbiology
Disease Outbreaks
Foodborne Diseases
03 medical and health sciences
Spatio-Temporal Analysis
0302 clinical medicine
medicine
Pulsed-field gel electrophoresis
Cluster (physics)
Humans
Prospective Studies
030212 general & internal medicine
United States Department of Agriculture
Disease Notification
Escherichia coli
Escherichia coli Infections
Gel electrophoresis
United States Food and Drug Administration
Outbreak
United States
Subtyping
Electrophoresis
Gel
Pulsed-Field
Gastroenteritis
Molecular Typing
Public Health Practice
Cluster size
Salmonella Food Poisoning
Animal Science and Zoology
Centers for Disease Control and Prevention
U.S
Food Science
Zdroj: Foodborne Pathogens and Disease. 13:674-678
ISSN: 1556-7125
1535-3141
Popis: Molecular subtyping of pathogens is critical for foodborne disease outbreak detection and investigation. Many clusters initially identified by pulsed-field gel electrophoresis (PFGE) are not confirmed as point-source outbreaks. We evaluated characteristics of clusters that can help prioritize investigations to maximize effective use of limited resources.A multiagency collaboration (FoodNet) collected data on Salmonella and Escherichia coli O157 clusters for 3 years. Cluster size, timing, extent, and nature of epidemiologic investigations were analyzed to determine associations with whether the cluster was identified as a confirmed outbreak.During the 3-year study period, 948 PFGE clusters were identified; 849 (90%) were Salmonella and 99 (10%) were E. coli O157. Of those, 192 (20%) were ultimately identified as outbreaks (154 [18%] of Salmonella and 38 [38%] of E. coli O157 clusters). Successful investigation was significantly associated with larger cluster size, more rapid submission of isolates (e.g., for Salmonella, 6 days for outbreaks vs. 8 days for nonoutbreaks) and PFGE result reporting to investigators (16 days vs. 29 days, respectively), and performance of analytic studies (completed in 33% of Salmonella outbreaks vs. 1% of nonoutbreaks) and environmental investigations (40% and 1%, respectively). Intervals between first and second cases in a cluster did not differ significantly between outbreaks and nonoutbreaks.Molecular subtyping of pathogens is a rapidly advancing technology, and successfully identifying outbreaks will vary by pathogen and methods used. Understanding criteria for successfully investigating outbreaks is critical for efficiently using limited resources.
Databáze: OpenAIRE
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