Effect of the substrate on neurofilament phosphorylation in mixed cultures of rat embryo spinal cord and dorsal root ganglia
| Autor: | Amico Bignami, Doris Dahl, Varda H. Gilad, L. Maggini |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Neurofilament
Neurite Central nervous system Intermediate Filaments Biology Epitopes chemistry.chemical_compound Developmental Neuroscience Pregnancy Laminin Ganglia Spinal medicine Animals Polylysine Phosphorylation Cells Cultured Antibodies Monoclonal Rats Inbred Strains Embryo Mammalian Spinal cord Immunohistochemistry Fibronectins Rats Cell biology medicine.anatomical_structure Spinal Cord chemistry Cell culture biology.protein Female Neuroscience Developmental Biology |
| Zdroj: | International Journal of Developmental Neuroscience. 10:111-119 |
| ISSN: | 1873-474X 0736-5748 |
| DOI: | 10.1016/0736-5748(92)90039-3 |
| Popis: | The effect of the substrate on neurofilament phosphorylation was studied in primary cultures of spinal cord and dorsal root ganglia dissociated from 15-day-old rat embryos. On polylysine and Primaria substrates, spinal cord neurons formed aggregates connected by bundles of neurites. (Primaria dishes have a modified plastic surface with a net positive charge). On both polylysine and Primaria substrates, spinal cord neurons were stained with neurofilament monoclonal antibodies reacting with phosphorylated epitopes appearing early in rat embryo development, i.e. soon after neurofilament expression. Conversely, immunoreactivity with antibodies recognizing late phosphorylation events was only observed on Primaria substrates. As reported by many investigators, fibronectin and laminin were excellent substrates for dorsal root ganglia neurons in culture. However, on both laminin and fibronectin substrates immunoreactivity with antibodies recognizing late phosphorylation events, was only observed on Primaria substrates. As reported by many investigators, fibronectin and laminin were excellent substrates for dorsal root ganglia neurons in culture. However, on both laminin and fibronectin substrates immunoreactivity with antibodies recognizing late phosphorylation events, only occurred after several days in culture, at a time when non-neuronal cells (mainly astrocytes) had formed a confluent monolayer. |
| Databáze: | OpenAIRE |
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