Enzymatic coupling of phenol vapors onto chitosan
| Autor: | Gregory F. Payne, Rafael Vazquez-Duhalt, Li-Qun Wu, Tianhong Chen, Kimberlee K. Wallace |
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| Rok vydání: | 2001 |
| Předmět: |
Time Factors
Ultraviolet Rays Tyrosinase chemistry.chemical_element Bioengineering Biocompatible Materials Chitin Applied Microbiology and Biotechnology Oxygen Chitosan chemistry.chemical_compound Cresols Adsorption parasitic diseases medicine Phenol Organic chemistry Phenols Monophenol Monooxygenase technology industry and agriculture food and beverages Cresol equipment and supplies Membrane chemistry Spectrophotometry Nuclear chemistry medicine.drug Biotechnology Disinfectants Protein Binding |
| Zdroj: | Biotechnology and bioengineering. 76(4) |
| ISSN: | 0006-3592 |
| Popis: | Phenols are important industrial chemicals, and because they can be volatile, also appear as air pollutants. We examined the potential of tyrosinase to react with the volatile phenol p-cresol. Three lines of evidence support the conclusion that volatile phenols react with tyrosinase and are coupled (i.e., chemisorbed) onto chitosan films. First, phenol-trapping studies indicated that p-cresol can be removed from vapors if the vapors are contacted with tyrosinase-coated chitosan films. Second, the ultraviolet absorbance of tyrosinase-coated chitosan films changes dramatically when they are contacted with cresol-containing vapors, whereas control films are unaffected by contacting with cresol vapors. Third, pressure measurements indicate that tyrosinase-coated chitosan films only react with cresol vapors if the oxygen cosubstrate is present. Additional studies demonstrate the potential of tyrosinase-coated chitosan films/membranes for the detection and removal of phenol vapors. |
| Databáze: | OpenAIRE |
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