Depletion of CPEB1 protects against oxidized LDL-induced endothelial apoptosis and inflammation though SIRT1/LOX-1 signalling pathway
| Autor: | Xiwen Liu, Suli Guo, Dexin Yin, Kaicheng Xu, Guanghao Ren, Yue Zhao |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Small interfering RNA Inflammation Apoptosis medicine.disease_cause 030226 pharmacology & pharmacy General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Sirtuin 1 Transcription (biology) medicine Human Umbilical Vein Endothelial Cells Humans Endothelium General Pharmacology Toxicology and Pharmaceutics mRNA Cleavage and Polyadenylation Factors biology Kinase Chemistry RNA General Medicine Atherosclerosis Scavenger Receptors Class E Cell biology Lipoproteins LDL Oxidative Stress 030104 developmental biology biology.protein lipids (amino acids peptides and proteins) medicine.symptom Oxidative stress Signal Transduction Transcription Factors |
| Zdroj: | Life sciences. 239 |
| ISSN: | 1879-0631 |
| Popis: | Atherosclerosis (AS) is a chronic inflammatory disease that results from Oxidized low-density lipoprotein (Ox-LDL) induced endothelial dysfunction. Cytoplasmic polyadenylation element binding protein 1 (CPEB1) is closely related to the development of epithelial cells, but the role of CPEB1 in AS remains unknown. The RNA and protein levels of CPEB1 expression are increased by Ox-LDL exposure, which is abrogated by c-Jun amino-terminal kinase (JNK) inhibitor SP600125. CPEB1 small interfering RNA (siRNA) suppressed the oxidative stress, inflammation, and apoptosis. Furthermore, CPEB1 siRNA enhanced the sirtuin 1 (SIRT1) transcription levels in Ox-LDL-treated HUVECs. Co-Immunoprecipitation (Co-IP) assay showed that CPEB1 siRNA declined the ubiquitination of SIRT1, and SIRT1 siRNA enhanced the Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), which were decreased by CPEB1 siRNA. In addition, LOX-1 and SIRT1 attenuated the protection of SIRT1 siRNA on Ox-LDL-induced oxidative stress. Therefore, our study revealed that CPEB1 depletion might play an anti-inflammatory and antiapoptotic role in Ox-LDL-induced apoptosis and inflammation though SIRT1/LOX-1 signalling pathway. |
| Databáze: | OpenAIRE |
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