Effects of Dietary Protein Intake on Cutaneous and Systemic Inflammation in Mice with Acute Experimental Psoriasis
| Autor: | Ari Waisman, Julia Ringen, Johannes Wild, Tanja Knopp, Thomas Münzel, Michael Molitor, Tabea Bieler, Annika Jurda, Rebecca Jung, Philip Wenzel, Susanne Karbach |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Low protein psoriasis-like skin disease Spleen High-protein diet Imiquimod Inflammation medicine.disease_cause Systemic inflammation Polymerase Chain Reaction Article Mice 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine protein diet Psoriasis medicine Animals TX341-641 Skin Nutrition and Dietetics business.industry Nutrition. Foods and food supply psoriasis Flow Cytometry medicine.disease Diet Mice Inbred C57BL imiquimod Disease Models Animal 030104 developmental biology medicine.anatomical_structure inflammation Immunology Dietary Proteins medicine.symptom Metabolic syndrome business Food Science medicine.drug |
| Zdroj: | Nutrients, Vol 13, Iss 1897, p 1897 (2021) Nutrients Volume 13 Issue 6 |
| ISSN: | 2072-6643 |
| Popis: | Background: Psoriasis is a systemic inflammatory disorder, primarily characterized by skin plaques. It is linked to co-morbidities including cardiovascular disease and metabolic syndrome. Several studies demonstrate that dietary habits can influence psoriasis development and severity. However, the effect of different dietary protein levels on psoriasis development and severity is poorly understood. In this study, we examine the influence of dietary protein on psoriasis-like skin disease in mice. Methods: We fed male C57BL/6J mice with regular, low protein and high protein chow for 4 weeks. Afterwards, we induced psoriasis-like skin disease by topical imiquimod (IMQ)-treatment on ear and back skin. The local cutaneous and systemic inflammatory response was investigated using flow cytometry analysis, histology and quantitative rt-PCR. Results: After 5 days of IMQ-treatment, both diets reduced bodyweight in mice, whereas only the high protein diet slightly aggravated IMQ-induced skin inflammation. IMQ-treatment induced infiltration of myeloid cells, neutrophils, and monocytes/macrophages into skin and spleen independently of diet. After IMQ-treatment, circulating neutrophils and reactive oxygen species were increased in mice on low and high protein diets. Conclusion: Different dietary protein levels had no striking effect on IMQ-induced psoriasis but aggravated the systemic pro-inflammatory phenotype. |
| Databáze: | OpenAIRE |
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