AP-1 (c-Fos/c-Jun) is required for corneal epithelial spreading
| Autor: | Kumi Shirai, Emiko Senba, Yuka Okada, Yoshitaka Ohnishi, Shizuya Saika, Masayasu Miyajima, Natsuko Hashizume, Osamu Yamanaka, Takeshi Miyamoto |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Pathology medicine.medical_specialty Stromal cell Proto-Oncogene Proteins c-jun Ratón Biology Organ culture c-Fos Mice Cellular and Molecular Neuroscience Organ Culture Techniques Cell Movement Cornea medicine Animals Corneal epithelium c-jun Epithelium Corneal Molecular biology Sensory Systems Epithelium Culture Media Mice Inbred C57BL Transcription Factor AP-1 Ophthalmology medicine.anatomical_structure biology.protein Female Proto-Oncogene Proteins c-fos |
| Zdroj: | Graefe's Archive for Clinical and Experimental Ophthalmology. 241:327-329 |
| ISSN: | 0721-832X |
| Popis: | Purpose. We previously demonstrated that the AP-1 components c-fos/c-jun are up-regulated in healing rat corneal epithelium in a relatively early phase following epithelial debridement, implicating the AP-1 function in the initiation of cell movement. To explore this hypothesis, we examined the effect of lack of c-Fos and c-Jun protein expressions on the spreading of corneal epithelium and in situ in organ culture. Antisense-oligonucleotide (AS) c-fos-null mice were used for this purpose. Methods. A rectangular piece of corneal tissue (2 x2 mm) was obtained from each eye of recently killed adult C57BL/6 mice and was incubated for 11 h in culture medium with 8 µM c-fos AS or c-jun AS probe. Sense probes were used for negative control. A rectangular section of corneal tissue was also obtained from each eye of c-fos -/-, c-fos +/- and c-fos +/+ mice and was organ-cultured for 11 h. The length of the path of epithelial spreading on stromal cut surface (both sides) was measured in hematoxylin-eosin-stained specimens. Data were analyzed by unpaired Student's t-test. Results. Addition of c-fos AS to the medium decreased the length of epithelial spread to 40.36% of that in the control with the S probe. Addition of c-jun AS decreased the length of epithelial spreading rate to 42.71% of control with S probe. Lacking c-Fos decreased the epithelial spreading to 17.73% of control data from c-fos +/- and c-fos +/+ mice. Conclusion. AP-1 (c-Fos/c-Jun) is required for the corneal epithelial spreading. |
| Databáze: | OpenAIRE |
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