Expression of glucose transporters (GLUT 1 and GLUT 4) in primary cultured rat adipocytes: Differential evolution with time and chronic insulin effect
| Autor: | Colette Guichard, Isabelle Hainault, Eric Hajduch, M Lavau, Michèle Guerre-Millo |
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| Rok vydání: | 1992 |
| Předmět: |
endocrine system
medicine.medical_specialty Time Factors Monosaccharide Transport Proteins endocrine system diseases medicine.medical_treatment Muscle Proteins Adipose tissue Biology Biochemistry chemistry.chemical_compound Insulin resistance Adipocyte Internal medicine Gene expression medicine Animals Insulin Molecular Biology Cells Cultured Glucose Transporter Type 1 Glucose Transporter Type 4 Glucose transporter nutritional and metabolic diseases Cell Biology medicine.disease Rats Rats Zucker carbohydrates (lipids) Endocrinology Adipose Tissue chemistry Cell culture hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Cellular Biochemistry. 49:251-258 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.240490307 |
| Popis: | We previously reported that in cultured adipose cell lines insulin increased selectively the expression of Glut 1, in contrast to in vivo regulation where variations in insulinemia have been shown to affect only GLUT 4. We have addressed here the question of the long-term regulation of GLUT 1 and GLUT 4 in fat cells by using primary cultures of rat adipocytes. Epididymal fat cells were isolated by collagenase and cultured 4 days in DMEM supplemented with BSA 1%, FCS 1%, and glucose 10 mM. GLUT 1 and GLUT 4 proteins were assessed in total cellular membranes by Western blotting, using specific antibodies against their respective C-terminal peptides. GLUT 1 steadily increased over culture time to reach at day 3, a level 3-fold higher than the initial value. In contrast, GLUT 4 decreased sharply and stabilized at day 3, at 30% of the initial value. The changes in GLUT 1 and GLUT 4 mRNAs with culture time were parallel to changes in the corresponding proteins, suggesting a pre-translational level of regulation. The expression of the lipogenic enzyme, fatty acid synthetase (FAS), highly expressed in fat cell, decreased over time following a pattern closely parallel to that of GLUT 4. Chronic exposure to insulin added at day 2 had no effect on GLUT 4 expression but increased the expression of GLUT 1 and FAS by 70% and 36%, respectively. Glucose consumption was stable over 4 days of culture, while lactate production increased from 24 to 36% of glucose utilization, in agreement with the loss in FAS. Glucose consumption increased only slightly with insulin (+160%), in good keeping with the low levels of expression of both GLUT 4 and FAS in these cultured cells. These data indicate that culture alters oppositely the expression of GLUT 1 and GLUT 4 in rat adipocytes and suggest that factor(s) other than insulin predominate in their regulation in vivo. |
| Databáze: | OpenAIRE |
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