Vitamin K1 accumulation in tobacco plants overexpressing bacterial genes involved in the biosynthesis of salicylic acid
| Autor: | Kamonchanok Sansuk, Robert Verpoorte, John F. Bol, Huub J. M. Linthorst, Marianne C. Verberne |
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| Rok vydání: | 2006 |
| Předmět: |
Vitamin
Cyclohexanecarboxylic Acids Transgene Nicotiana tabacum Chorismic Acid Carbon-Oxygen Lyases Bioengineering Applied Microbiology and Biotechnology chemistry.chemical_compound Biosynthesis Bacterial Proteins Cyclohexenes Tobacco Chorismic acid Intramolecular Transferases biology food and beverages General Medicine Vitamin K 1 biology.organism_classification Plants Genetically Modified Recombinant Proteins Biosynthetic Pathways Chloroplast chemistry Biochemistry Isochorismate synthase biology.protein Salicylic Acid Salicylic acid Biotechnology |
| Zdroj: | Journal of biotechnology. 128(1) |
| ISSN: | 0168-1656 |
| Popis: | Phylloquinone (Vitamin K(1)) is an essential component of the photosynthetic electron transfer. As isochorismate is required for the biosynthesis of Vitamin K(1), isochorismate synthase (ICS) activity is expected to be present in all green plants. In bacteria salicylic acid (SA) is synthesized via a two step pathway involving ICS and isochorismate pyruvate lyase (IPL). The effect of the introduction in tobacco plants of the bacterial ICS and IPL genes on the endogenous isochorismate pathway was investigated. Transgenic tobacco plants in which IPL was targeted to the chloroplast suffered severe growth retardation and had low Vitamin K(1) content. Probably because isochorismate was channeled towards SA production, the plants were no longer able to produce normal levels of Vitamin K(1). Transgenic tobacco plants in which the bacterial ICS was present in the chloroplast showed higher Vitamin K(1) contents than wild type plants. |
| Databáze: | OpenAIRE |
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