| Autor: |
Stow, Emily C., Baddoo, Melody, LaRosa, Alexis J., LaCoste, Dawn, Deininger, Prescott, Belancio, Victoria |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.20678863.v1 |
| Popis: |
Additional file 9. Analysis of L1 mRNA expression in human testes with bulk RNA-Seq and scRNA-Seq. A. The L1 FPKM values for L1 loci listed on the X-axis are shown for two 20 yo human testis bulk RNA-Seq samples. The number of expressed L1 loci shared between the two replicates is shown in the Venn diagram with the percentage of shared expressed loci indicated above the diagram. B. The L1 RPM values for L1 loci listed on the X-axis are shown for a 24 yo and a 25 yo human testis scRNA-Seq sample. The number of expressed L1 loci shared between the two replicates is shown in the Venn diagram with the percentage of shared expressed loci indicated above the diagram. C. The million mapped reads per cell for all cell types in the 24 yo scRNA-Seq dataset is shown in the scatter plot. Statistics are shown for sperm cells and sperm progenitor cell types only. D. The L1 mRNA expression level per cell for all 24 yo testis cell types is shown in the scatter plot. E. The number of expressed L1 loci per cell for all 24 yo testis cell types is shown in the scatter plot. F. The million mapped reads per cell for all cell types in the 24 yo scRNA-Seq dataset is shown in the scatter plot. Statistics are shown for sperm cells and sperm progenitor cell types only. G. The L1 mRNA expression level per cell for all 25 yo testis cell types is shown in the scatter plot. H. The number of expressed L1 loci per cell for all 25 yo testis cell types is shown in the scatter plot. I. The L1 RPM values for L1 loci indicated on the X-axis are shown for each sperm cell type in the 25 yo human testis sample. The Venn Diagram shows the number of expressed L1 loci shared by the sperm cell types with the percentage of shared expressed L1 loci indicated above the diagram. J. The L1 FPKM values for bulk RNA-Seq (left y-axis) and L1 RPM values for scRNA-Seq (right y-axis) of human testis are shown in the dot-plot. Orange circles indicate L1 loci with detected expression in the bulk dataset that were not detected to be expressed in scRNA-Seq. The nested Venn diagram shows the number of shared expressed L1 loci from the bulk and scRNA-Seq datasets. K. The violin plot shows the FPKM values for expressed L1 loci unique to the bulk dataset and those shared between bulk and scRNA-Seq (t-test, **,< 0.0021). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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