An immuno polymerase chain reaction screen for the detection of CJC‐1295 and other growth‐hormone‐releasing hormone analogs in equine plasma
| Autor: | Katherine Ganio, Mark Timms, Simon R. Bailey, Grace Forbes, Rohan Steel |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.drug_class Pharmaceutical Science Peptide Growth Hormone-Releasing Hormone Monoclonal antibody Polymerase Chain Reaction 01 natural sciences Analytical Chemistry 03 medical and health sciences Limit of Detection medicine Animals Environmental Chemistry Secretion Horses Spectroscopy Immunoassay chemistry.chemical_classification biology medicine.diagnostic_test Chemistry 010401 analytical chemistry Antibodies Monoclonal Surface Plasmon Resonance Growth hormone–releasing hormone CJC-1295 Blood proteins Peptide Fragments 0104 chemical sciences Substance Abuse Detection 030104 developmental biology Biochemistry biology.protein Antibody |
| Zdroj: | Drug Testing and Analysis. 11:804-812 |
| ISSN: | 1942-7611 1942-7603 |
| Popis: | CJC-1295 is a 30 amino acid peptide-based drug that stimulates the release of growth hormone (GH) from the pituitary gland. It is unique among performance-enhancing peptides due to the presence of a reactive maleimidopropionic acid group that covalently links the peptide to free thiols on the surface of plasma proteins. Once conjugated, CJC-1295 remains active in the bloodstream for significantly longer than non-conjugated peptide-based drugs that are rapidly excreted. Conjugation of CJC-1295 to plasma proteins prevents its detection by top-down mass-spectrometry-based peptide screening protocols as it effectively becomes a macromolecular protein with an undefined molecular weight. Using a pair of monoclonal antibodies raised against the CJC-1295 peptide, we present an immuno-polymerase chain reaction (I-PCR) assay that is capable of detecting the CJC-1295-protein conjugate at concentrations down to 0.8 pg/mL. Detection of endogenous equine GHRH necessitated a screening threshold for CJC-1295 in equine plasma of 50 pg/mL. The effectiveness of the assay for controlling the illicit use of CJC-1295 was confirmed in equine blood samples after administration in thoroughbred race horses. |
| Databáze: | OpenAIRE |
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