Cryo-EM samples of gas-phase purified protein assemblies using native electrospray ion-beam deposition

Autor:	Tim K. Esser, Jan Böhning, Paul Fremdling, Tanmay Bharat, Joseph Gault, Stephan Rauschenbach
Rok vydání:	2022
Předmět:	Ions Cryoelectron Microscopy Humans Proteins Physical and Theoretical Chemistry Inflammatory Bowel Diseases Mass Spectrometry Specimen Handling
Zdroj:	Faraday Discussions. 240:67-80
ISSN:	1364-5498 1359-6640
DOI:	10.1039/d2fd00065b
Popis:	An increasing number of studies on biomolecular function indirectly combine mass spectrometry (MS) with imaging techniques such as cryo electron microscopy (cryo-EM). This approach allows information on the homogeneity, stoichiometry, shape, and interactions of native protein complexes to be obtained, complementary to high-resolution protein structures. We have recently demonstrated TEM sample preparation
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51c02f8ea6e1fc6e0e69041999fe2839 https://doi.org/10.1039/d2fd00065b Zobrazit plný text záznamu