An oligosaccharide-marker approach to quantify specific polysaccharides in herbal formula by LC-qTOF-MS: Danggui Buxue Tang, a case study
| Autor: | Hui-Yuan Cheng, Ai-Ping Lyu, Li-Shuang Zhou, Li-Feng Li, Ji-Xia Zhang, Dik-Lung Ma, Quan-Bin Han, Hongming Zheng, Wan-Rong Bao, Su-Ping Bai, Tin-Long Wong, Hau-Yee Fung, Chung-Hang Leung, Ge Zhang, Quan-Wei Zhang, Zhaoxiang Bian |
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| Rok vydání: | 2019 |
| Předmět: |
Quality Control
Spectrometry Mass Electrospray Ionization Chemistry Pharmaceutical Clinical Biochemistry Pharmaceutical Science Oligosaccharides Decoction Polysaccharide 01 natural sciences Analytical Chemistry Lc qtof ms Spike recovery Liquid chromatography–mass spectrometry Tandem Mass Spectrometry Drug Discovery Spectroscopy Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography Danggui Buxue Tang 010405 organic chemistry Quality assessment 010401 analytical chemistry Reproducibility of Results Oligosaccharide Reference Standards 0104 chemical sciences chemistry Drugs Chinese Herbal |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 185 |
| ISSN: | 1873-264X |
| Popis: | Polysaccharides have broad bioactivities and are major components of water decoction of herb formulae. However, the quality control of polysaccharides remains a challenge. Oligosaccharide-fragment approach has been considered in elucidating chemical structures of polysaccharides, but never been used for quantitation. Using reference chemicals and a real sample Danggui Buxue Tang (DBT) in this study, an oligosaccharide-marker approach was established to quantify specific polysaccharides. Firstly, linear relationships between parent polysaccharides and hydrolysis-produced daughter oligosaccharides were verified using reference polysaccharides. Then in case of DBT, two fluorescence-labeled oligosaccharides with high specificity to individual parent polysaccharides were selected as markers. They were easily isolated and identified. Their potential in quantification of parent polysaccharides were satisfactorily validated in terms of linearity (r≥0.99), repeatability (RSD ≤ 8.4 %), and spike recovery (≥80 %). This method could be a promising approach for quality assessment of polysaccharides in herbal formulae. |
| Databáze: | OpenAIRE |
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