Use of In Vivo and In Vitro Systems to Select Leishmania amazonensis Expressing Green Fluorescent Protein
| Autor: | Fabio T. M. Costa, Bartira Rossi-Bergmann, Marjorie de Assis Golim, Solange dos Santos Costa, Selma Giorgio |
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| Přispěvatelé: | Universidade Estadual de Campinas (UNICAMP), Universidade Estadual Paulista (Unesp), Universidade Federal do Rio de Janeiro (UFRJ) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
green fluorescent protein
viruses Green Fluorescent Proteins Leishmania mexicana Leishmaniasis Cutaneous macrophage Biology Green fluorescent protein Microbiology Host-Parasite Interactions Mice Plasmid Cutaneous leishmaniasis In vivo medicine Parasite hosting Animals Amebicides Amastigote Mice Inbred BALB C Luminescent Agents Organisms Genetically Modified Aminoglycoside biochemical phenomena metabolism and nutrition medicine.disease Flow Cytometry In vitro Infectious Diseases Spectrometry Fluorescence embryonic structures Macrophages Peritoneal geneticin Parasitology Original Article Gentamicins Leishmania amazonensis |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP The Korean Journal of Parasitology |
| ISSN: | 0002-9904 |
| Popis: | Made available in DSpace on 2013-09-27T14:54:23Z (GMT). No. of bitstreams: 1 WOS000299048000003.pdf: 1427401 bytes, checksum: c37a804c88f8f546fb85d5bb4f94f361 (MD5) Previous issue date: 2011-12-01 Made available in DSpace on 2013-09-30T19:24:36Z (GMT). No. of bitstreams: 1 WOS000299048000003.pdf: 1427401 bytes, checksum: c37a804c88f8f546fb85d5bb4f94f361 (MD5) Previous issue date: 2011-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:34:41Z No. of bitstreams: 1 WOS000299048000003.pdf: 1427401 bytes, checksum: c37a804c88f8f546fb85d5bb4f94f361 (MD5) Made available in DSpace on 2014-05-20T15:34:41Z (GMT). No. of bitstreams: 1 WOS000299048000003.pdf: 1427401 bytes, checksum: c37a804c88f8f546fb85d5bb4f94f361 (MD5) Previous issue date: 2011-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Various Leishmania species were engineered with green fluorescent protein (GFP) using episomal vectors that encoded an antibiotic resistance gene, such as aminoglycoside geneticin sulphate (G418). Most reports of GFP-Leishmania have used the flagellated extracellular promastigote, the stage of parasite detected in the midgut of the sandfly vector; fewer studies have been performed with amastigotes, the stage of parasite detected in mammals. In this study, comparisons were made regarding the efficiency for in vitro G418 selection of GFP-Leishmania amazonensis promastigotes and amastigotes and the use of in vivo G418 selection. The GFP-promastigotes retained episomal plasmid for a prolonged period and G418 treatment was necessary and efficient for in vitro selection. In contrast, GFP-amastigotes showed low retention of the episomal plasmid in the absence of G418 selection and low sensitivity to antibiotics in vitro. The use of protocols for G418 selection using infected BALB/c mice also indicated low sensitivity to antibiotics against amastigotes in cutaneous lesions. Univ Estadual Campinas, Dept Anim Biol, Inst Biol, BR-13083970 São Paulo, Brazil Univ Estadual Campinas, Dept Genet Evolut & Bioagents, Inst Biol, BR-13083970 São Paulo, Brazil Univ Estadual Paulista, Blood Ctr, Fac Med, Sch Med, São Paulo, Brazil Univ Fed Rio de Janeiro, Carlos Chagas Filho Inst, Rio de Janeiro, Brazil Univ Estadual Paulista, Blood Ctr, Fac Med, Sch Med, São Paulo, Brazil |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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