Metabolism of carbohydrate derivatives by Pseudomonas acidovorans
| Autor: | J R Taylor, H E Heath, M L Rogers, M H Wettermark |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Glycerol
Pyruvate Kinase Dehydrogenase Biology Gluconates Microbiology chemistry.chemical_compound Glucose dehydrogenase Pseudomonas Phosphogluconate dehydrogenase Molecular Biology Phosphoglycerate Mutase Glucokinase Glyceraldehyde-3-Phosphate Dehydrogenases Metabolism NAD Phosphoglycerate Kinase Glucose Biochemistry chemistry Phosphopyruvate Hydratase Mutation NAD+ kinase Pyruvate kinase Research Article |
| Zdroj: | Journal of Bacteriology. 138:418-424 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Wild-type Pseudomonas acidovorans strain A1 was unable to grow on glycerol or glucose as sole source of carbon and energy although it grew well on gluconate. Spontaneous glycerol-positive mutants, which apparently had become permeable to glycerol, were readily isolated, but glucose-positive mutants did not occur. P. acidovorans lacked glucose dehydrogenase and glucokinase, which were sufficient to account for its inability to grow on glucose. Gluconate was degraded exclusively via a noncoordinately induced Entner-Doudoroff pathway. Phosphogluconate dehydrogenase was undetectable. In contrast to P. aeruginosa, P. acidovorans possessed a single glyceraldehyde-phosphate dehydrogenase activity, which was NAD+ specific and constitutive, and an inducible pyruvate kinase. Moreover, growth of glycerol-positive strain K2 on glycerol did not induce any of the enzymes related to metabolism of hexosephosphate derivatives as occurs in fluorescent pseudomonads. |
| Databáze: | OpenAIRE |
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