LncRNA-ROR/microRNA-185-3p/YAP1 axis exerts function in biological characteristics of osteosarcoma cells
| Autor: | Pei Qi, Haiyang Yu, Shijie Chen, Minghua Hu, Yan Huang, Xingchang Fu, Ruping Zheng, Zhiyu Ding, Yi Peng, Jianlong Wang, Jinglei Miao, Jinsong Li, Yuezhan Li, Weiguo Wang, Shuang Zhi |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male musculoskeletal diseases 0106 biological sciences Adolescent Cell Apoptosis Biology 01 natural sciences Metastasis Mice 03 medical and health sciences Cell Line Tumor microRNA Genetics medicine Animals Humans Child Cell Proliferation 030304 developmental biology YAP1 Mice Inbred BALB C Osteosarcoma 0303 health sciences Gene knockdown YAP-Signaling Proteins Transfection Middle Aged medicine.disease MicroRNAs medicine.anatomical_structure Cancer research Female RNA Long Noncoding 010606 plant biology & botany |
| Zdroj: | Genomics. 113:450-461 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/j.ygeno.2020.09.009 |
| Popis: | Aim The co-expression network of long non-coding RNA ROR (lncRNA-ROR) and microRNA-185-3p (miR-185-3p) has not been focused on osteosarcoma. Therein, this work was initiated to uncover lncRNA-ROR and miR-185-3p functions in osteosarcoma. Methods LncRNA-ROR, miR-185-3p and Yes-associated protein 1 (YAP1) expression in osteosarcoma tissues and cells were detected. The screened cells (MG63 and U2OS) were transfected with decreased and/or increased lncRNA-ROR and miR-185-3p to explore osteosarcoma progression. Tumor growth was detected by tumor xenografts in mice. Results Up-regulated lncRNA-ROR and YAP1 and down-regulated miR-185-3p were found in osteosarcoma. LncRNA ROR knockdown or miR-185-3p overexpression inhibited osteosarcoma cell progression while lncRNA ROR elevation or miR-185-3p inhibition presented the opposite effects. Function of lncRNA ROR was rescued by miR-185-3p and regulated the growth and metastasis of osteosarcoma cells via modulating YAP1, the target gene of miR-185-3p. Conclusion This work illustrates that lncRNA-ROR down-regulation or miR-185-3p up-regulation inhibits osteosarcoma progression via YAP1 repression. |
| Databáze: | OpenAIRE |
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