Three Component Composite Scaffolds Based on PCL, Hydroxyapatite, and L-Lysine Obtained in TIPS-SL: Bioactive Material for Bone Tissue Engineering

Autor:	Aleksandra Korbut, Marcin Włodarczyk, Karolina Rudnicka, Aleksandra Szwed, Przemysław Płociński, Monika Biernat, Paulina Tymowicz-Grzyb, Martyna Michalska, Natalia Karska, Sylwia Rodziewicz-Motowidło, Konrad Szustakiewicz
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	Bone Regeneration QH301-705.5 Polyesters Biocompatible Materials Article Catalysis Cell Line Inorganic Chemistry stomatognathic system foam scaffolds Humans polymers PCL cell adhesion L-Lysine Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy Cell Proliferation Osteoblasts Tissue Engineering Tissue Scaffolds Lysine Organic Chemistry technology industry and agriculture General Medicine Computer Science Applications Chemistry Durapatite
Zdroj:	International Journal of Molecular Sciences; Volume 22; Issue 24; Pages: 13589 International Journal of Molecular Sciences, Vol 22, Iss 13589, p 13589 (2021) International Journal of Molecular Sciences
ISSN:	1422-0067
DOI:	10.3390/ijms222413589
Popis:	In this research, we describe the properties of three-component composite foam scaffolds based on poly(ε-caprolactone) (PCL) as a matrix and hydroxyapatite whiskers (HAP) and L-Lysine as fillers (PCL/HAP/Lys with wt% ratio 50/48/2). The scaffolds were prepared using a thermally induced phase separation technique supported by salt leaching (TIPS-SL). All materials were precisely characterized: porosity, density, water uptake, wettability, DSC, and TGA measurements and compression tests were carried out. The microstructure of the obtained scaffolds was analyzed via SEM. It was found that the PCL/HAP/Lys scaffold has a 45% higher Young’s modulus and better wettability compared to the PCL/HAP system. At the same time, the porosity of the system was ~90%. The osteoblast hFOB 1.19 cell response was also investigated in osteogenic conditions (39 °C) and the cytokine release profile of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α was determined. Modification of PCL scaffolds with HAP and L-Lysine significantly improved the proliferation of pre-osteoblasts cultured on such materials.
Databáze:	OpenAIRE
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