Chemopreventive Efficacy of Ginger Extract (Zingiber officinale)
| Autor: | M. Noor Azian, W. N. Wan Zurinah, Harliansyah Harliansyah, M. Y. Yasmin Anum |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | ISCC (Indonesian Journal of Cancer Chemoprevention), Vol 1, Iss 2, Pp 118-123 (2010) |
| ISSN: | 2355-8989 2088-0197 |
| DOI: | 10.14499/indonesianjcanchemoprev1iss2pp118-123 |
| Popis: | The liver cells were cultured in the presence of ginger extract at various concentrations (0-1 mg /ml) for 24 h and the cells viability and proliferation rate were evaluated by MTS and BrdU assays, while apoptosis was evaluated by colorimetric determination of caspase 8 and 3 activities. Ginger extract exhibited a dose dependent inhibition of viability and proliferation of WRL-68, HLE and HepG2 cells with IC50 of 569.69 ± 7.99 µg/ml, 389.71 ± 26.56 µg/ml and 358.71 ± 17.12 µg/ml respectively. Ginger extract induced apoptosis through activation of caspase-8 and 3 in a dose dependent pattern for all cells at concentration ranging from 0-500 mg/ml. We found that antiproliferative effect of ginger extract could be associated with induction of apoptosis as shown by increased activities of caspase 8 and 3.The results from this study suggest that ginger extract has chemopreventive properties against hepatoma cells HepG2 and HLE by inhibiting cellular proliferation and inducing apoptosis.Keywords : antiproliferation, apoptosis, caspases, Zingiber officinale |
| Databáze: | OpenAIRE |
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