Protection of vascular basement membrane and microcirculation from elastase-induced damage with a fluorinated beta-lactam derivative
| Autor: | Roger Joyeau, R Kobaiter, Michèle Reboud-Ravaux, Michel Wakselman, Jean-Louis Maillard, Catherine Favreau, I. Vergely |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Injections Intradermal Renal glomerulus Clinical Biochemistry Guinea Pigs Kidney Glomerulus Molecular Sequence Data Vascular permeability Hemorrhage Antigen-Antibody Complex Pharmacology Biochemistry Skin Diseases Basement Membrane Capillary Permeability medicine Arthus Reaction Animals Humans Amino Acid Sequence Pancreatic elastase Basement membrane biology Pancreatic Elastase Arthus reaction Chemistry Glomerular basement membrane Biochemistry (medical) Elastase General Medicine medicine.disease medicine.anatomical_structure biology.protein Azetidines Cattle Female Leukocyte Elastase Elastin |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 213(1-3) |
| ISSN: | 0009-8981 |
| Popis: | N-(2-chloromethylphenyl) 3,3-difluoroazetidin-2-one (AA 231-1), a specific suicide-type inhibitor of elastase which is known to suppress the lysis of chromogenic oligopeptides, elastin and elastic fibers, is effective also in preventing the degradation of the vascular basement membrane. The degradation of porcine glomerular basement membrane by purified human leukocyte elastase (HLE), was reduced in proportion of inhibitor dose (8.3 microM for 50% inhibition). It is noteworthy that there was no reduction of the inhibitory effect when the addition of AA 231-1 was delayed for 1 h after the addition of the enzyme to the substrate. In the guinea pig, reduction of the dermal microhemorrhage due to HLE was related to the dose of inhibitor and to its preincubation time with HLE before intradermal injection. The inflammatory hemorrhage associated with the Arthus skin reaction was moderately depressed by AA 231-1 in situ. A part of the vascular permeability induced by HLE also responded to the inhibitor. In spite of the tissular diffusion and the time-dependence parameters which restrict responsiveness of elastase to AA 231-1 in vivo this biochemical compound should be helpful in the study and possibly the cure of vascular injury related to elastase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|