Dysregulation of mannose-6-phosphate–dependent cholesterol homeostasis in acinar cells mediates pancreatitis
| Autor: | Eszter T. Vegh, Natalia Shalbueva, Zoltán Rakonczay, Dustin L. Dillon, Nicholas O. Davidson, Stephen J. Pandol, Anna S. Gukovskaya, Olga A. Mareninova, David W. Dawson, Sudarshan R. Malla, Steven J. Bensinger, Carli J. Wightman, Toshimasa Takahashi, Yan Xie, Herbert Y. Gaisano, Samuel W. French, Fred S. Gorelick, Ilya Gukovsky |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Knockout
Immunology Transferases (Other Substituted Phosphate Groups) Mannose 6-phosphate Acinar Cells Medical and Health Sciences Oral and gastrointestinal chemistry.chemical_compound Mice Rare Diseases Downregulation and upregulation 03.01.07. Fiziológia (benne citológia) Autophagy Acinar cell medicine 2.1 Biological and endogenous factors Animals Humans Aetiology Trypsinogen activation Pancreas Mice Knockout Mannosephosphates Animal Cholesterol Gastroenterology Cell Biology General Medicine medicine.disease Pancreas Exocrine Mitochondria Cell biology Disease Models Animal chemistry Pancreatitis Disease Models Lysosomes Digestive Diseases Exocrine Homeostasis Research Article |
| Zdroj: | J Clin Invest The Journal of clinical investigation, vol 131, iss 15 |
| Popis: | Disordered lysosomal/autophagy pathways initiate and drive pancreatitis, but the underlying mechanisms and links to disease pathology are poorly understood. Here, we show that the mannose-6-phosphate (M6P) pathway of hydrolase delivery to lysosomes critically regulates pancreatic acinar cell cholesterol metabolism. Ablation of the Gnptab gene encoding a key enzyme in the M6P pathway disrupted acinar cell cholesterol turnover, causing accumulation of nonesterified cholesterol in lysosomes/autolysosomes, its depletion in the plasma membrane, and upregulation of cholesterol synthesis and uptake. We found similar dysregulation of acinar cell cholesterol, and a decrease in GNPTAB levels, in both WT experimental pancreatitis and human disease. The mechanisms mediating pancreatic cholesterol dyshomeostasis in Gnptab(–/–) and experimental models involve a disordered endolysosomal system, resulting in impaired cholesterol transport through lysosomes and blockage of autophagic flux. By contrast, in Gnptab(–/–) liver the endolysosomal system and cholesterol homeostasis were largely unaffected. Gnptab(–/–) mice developed spontaneous pancreatitis. Normalization of cholesterol metabolism by pharmacologic means alleviated responses of experimental pancreatitis, particularly trypsinogen activation, the disease hallmark. The results reveal the essential role of the M6P pathway in maintaining exocrine pancreas homeostasis and function, and implicate cholesterol disordering in the pathogenesis of pancreatitis. |
| Databáze: | OpenAIRE |
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