| Autor: |
Deng, Chunhao, Zhang, Zhaoying, Xu, Faxiang, Xu, Jiaqi, Ren, Zhili, Godoy-Parejo, Carlos, Xiao, Xia, Liu, Weiwei, Zhou, Zhou, Chen, Guokai |
| Rok vydání: |
2022 |
| Předmět: |
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| DOI: |
10.6084/m9.figshare.19396209.v1 |
| Popis: |
Additional file 2: Figure S2. T3 is compatible with long-term hESC maintenance in E8 medium. Related to Figure 2. A. Effect of T3 on pluripotency in H9 hESCs. H9 cells were maintained in E8 medium with or without T3 (500nM) for five passages, and the expression of pluripotency markers NANOG and POU5F1 were examined by real time PCR. GAPDH was used as internal control and gene expression was normalized to the level in E8 culture. B. Morphology of H9 hESCs cultured with or without T3 for five passages. Scale bar, 200��m. C. Effect of T3 on pluripotency in NL-1 hiPSC. NL-1 cells were maintained in E8 medium with or without T3 (500nM) for five passages, and the expression of pluripotency markers NANOG and POU5F1 were examined by real time PCR. GAPDH was used as internal control and gene expression was normalized to the level in E8 culture. D. Morphology of NL-1 hiPSCs cultured with or without T3 for five passages. Scale bar, 200��m. E. T3 enhances hESC mitochondrial spare respiration capacity |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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